Recombinant Cynomolgus Monkey B7-H2 (Catalog # 9327-B7)co-stimulates IL-4 secretion by D10.G4.1 mouse helper T cells in the presenceof anti-CD3 antibody. The ED50 for this effect is 0.15-0.9 μg/mL.
also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an
approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory
molecules (1). Mature cynomolgus B7-H2 consists of a 226 amino acid (aa) extracellular
domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane
segment, and a 25 aa cytoplasmic domain (2, 3). Within the ECD, cynomolgus
B7-H2 shares 94%, 50%, and 54% aa sequence identity with human, mouse, and rat
B7-H2, respectively. Alternative splicing generates a long isoform that carries
a 10 aa substitution for the 3 C-terminal residues in humans and a 27 aa
substitution for the 2 C-terminal residues in mouse. B7-H2 is expressed on antigen
presenting cells such as B cells, macrophages, monocytes, and dendritic cells (2-6). B7-H2 binds to ICOS on activated T cells, leading to both
positive and negative effects on immune responses including its own
down-regulation (2, 4, 7). Mouse and human B7-H2 exhibit cross-species binding
to ICOS (3, 6). The B7-H2 interaction with ICOS is co-stimulatory for T cell
proliferation as well as the development of B cells, plasma cells, follicular
helper T cells (Tfh) and germinal centers (2-4, 8, 9). In human but
not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction
with CD28 can co-stimulate both human and mouse naïve T cells and regulatory T cells (Treg) (6). B7-H2 contributes to the development of allergic asthma by
enhancing Th2 biased immune responses, limiting Th17 responses, and promoting
eosinophilic infiltration into the lung (8, 10, 11). Its activation of ICOS on
Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the
development of inhalational tolerance, and impairs anti-tumor immunity (5, 12, 13). In contrast, its ligation of ICOS on Tfh cells can increase the severity
of autoimmune symptoms (9). A soluble form of human B7-H2 is elevated in the
circulation of patients with active systemic lupus erythematosus (14).In the
thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes
their proliferation and production of thryoid hormones (15). B7-H2 and ICOS
expressed on ILC2 cells. B7-H2/ICOS interaction promoted cytokine production
and survival in ILC2 cells through STAT5, suggesting that B7-H2/ICOS signaling
pathway is critically involved in ILC2 function and homeostasis (16).
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