>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Human IgG
is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey Fc gamma RI/CD64 that produces 50% of the optimal binding response is approximately 1-5 ng/mL.
Human embryonic kidney cell, HEK293-derived Val11-Pro288, with a C-terminal 6-His tag
When Human IgG is immobilized at 0.5 μg/mL, 100 μL/well,Recombinant Cynomolgus Monkey Fc gamma RI/CD64 (Catalog # 9239-FC) binds withan ED50 of 1-5 ng/mL.
Background: Fc gamma RI/CD64
Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1, 2). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8 -10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1, 3). Mature cynomolgus Fc gamma RI consists of a 277 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RI shares 95%, 72%, and 66% aa sequence identity with human, mouse, and rat Fc gamma RI, respectively. It binds cynomolgus IgG subclasses 1-4 as well as human IgG 1, 3, and 4 (4, 5). It delivers an activating signal via the associated Fc R gamma accessory chain (6). Fc gamma RI is expressed constitutively on monocytes, macrophages, and monocyte-derived dendritic cells and can be induced on neutrophils, eosinophils, mast cells, and glomerular mesangial cells (1, 3). Its expression is up-regulated during bacterial infections and sepsis.
Chenoweth, A.M. et al. (2015) Immunol. Rev. 268:175.
Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
CD64 antigen; CD64; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FCRI; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I
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