Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein, CF

R&D Systems | Catalog # 9239-FC

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein (9239-FC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_001270969

Applications

Bioactivity
View all Fc gamma RI/CD64 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RI/CD64 protein
Val11-Pro288, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val11

Predicted Molecular Mass

32 kDa

SDS-PAGE

38-46 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human IgG is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey Fc gamma  RI/CD64 that produces 50% of the optimal binding response is approximately 1-5 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein, CF

Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein Bioactivity

Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein Bioactivity

When Human IgG is immobilized at 0.5 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey Fc gamma RI/CD64 (Catalog # 9239-FC) binds with an ED50 of 1-5 ng/mL.

Formulation, Preparation, and Storage

9239-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1, 2). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI/CD64, Fc gamma  RII/CD32, and Fc gamma  RIII/CD16. Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8 -10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1, 3). Mature cynomolgus Fc gamma RI consists of a 277 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RI shares 95%, 72%, and 66% aa sequence identity with human, mouse, and rat Fc gamma RI, respectively. It binds cynomolgus IgG subclasses 1-4 as well as human IgG 1, 3, and 4 (4, 5). It delivers an activating signal via the associated Fc R gamma accessory chain (6). Fc gamma RI is expressed constitutively on monocytes, macrophages, and monocyte-derived dendritic cells and can be induced on neutrophils, eosinophils, mast cells, and glomerular mesangial cells (1, 3). Its expression is up-regulated during bacterial infections and sepsis.

References

  1. Chenoweth, A.M. et al. (2015) Immunol. Rev. 268:175.
  2. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Warncke, M. et al. (2012) J. Immunol. 188:4405.
  5. Nguyen, D.C. et al. (2014) Immunogenetics 66:361.
  6. van Vugt, M.J. et al. (1996) Blood 87:3593.

Long Name

Fc gamma Receptor I

Alternate Names

CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA

Entrez Gene IDs

2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)

Gene Symbol

FCGR1A

UniProt

NP_001270969

Additional Fc gamma RI/CD64 Products

Product Documents for Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein, CF

For research use only

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