Recombinant Cynomolgus Monkey Fc gamma RI/CD64 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey Fc gamma  RI/CD64 that produces 50% of the optimal binding response is approximately 1-5 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Val11-Pro288, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Val11
  • Predicted Molecular Mass
    32 kDa
  • SDS-PAGE
    38-46 kDa, reducing conditions
9239-FC
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Human IgG is immobilized at 0.5 μg/mL, 100 μL/well,Recombinant Cynomolgus Monkey Fc gamma RI/CD64 (Catalog # 9239-FC) binds withan ED50 of
1-5 ng/mL.
Background: Fc gamma RI/CD64
Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1, 2). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI/CD64, Fc gamma  RII/CD32, and Fc gamma  RIII/CD16. Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8 -10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1, 3). Mature cynomolgus Fc gamma RI consists of a 277 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RI shares 95%, 72%, and 66% aa sequence identity with human, mouse, and rat Fc gamma RI, respectively. It binds cynomolgus IgG subclasses 1-4 as well as human IgG 1, 3, and 4 (4, 5). It delivers an activating signal via the associated Fc R gamma accessory chain (6). Fc gamma RI is expressed constitutively on monocytes, macrophages, and monocyte-derived dendritic cells and can be induced on neutrophils, eosinophils, mast cells, and glomerular mesangial cells (1, 3). Its expression is up-regulated during bacterial infections and sepsis.
  • References:
    1. Chenoweth, A.M. et al. (2015) Immunol. Rev. 268:175.
    2. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
    3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
    4. Warncke, M. et al. (2012) J. Immunol. 88:4405.
    5. Nguyen, D.C. et al. (2014) Immunogenetics 66:361.
    6. van Vugt, M.J. et al. (1996) Blood 87:3593.
  • Long Name:
    Fc gamma Receptor I
  • Entrez Gene IDs:
    2209 (Human); 14129 (Mouse); 102147198 (Cynomolgus Monkey)
  • Alternate Names:
    CD64 antigen; CD64; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI/CD64; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FCGR1A; FcgRI; FCRI; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I
Related Research Areas
Primary Antibodies
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His Tag Antibody

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His Tag Alexa Fluor® 488-conjugated Antibody

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His Tag Alexa Fluor® 700-conjugated Antibody

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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

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