Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF Summary
|Cynomolgus Monkey BCMA (Met1-Ile54) Accession # XP_005591343||IEGRMD||Human IgG 1 (Pro100-Lys330)||N-terminus||C-terminus|
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Chimera(Catalog # 10029-BC) inhibits Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP) mediated proliferation of anti-IgM stimulated mouse B cells. The ED50 for this effectis 30-180 ng/mL.
2 μg/lane of Recombinant Cynomolgus Monkey BCMA/TNFRSF17 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 37‑43 kDa and 75‑85 kDa, respectively.
BCMA, B cell maturation antigen, also known as Tumor Necrosis Factor Receptor Superfamily member 17 (gene name TNSFR17), is a member of the TNFR superfamily, due to the presence of its TNFR motif (1). BCMA is a type III membrane protein containing one extracellular cysteine rich domain, a transmembrane domain, and an intracellular domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily (2, 3). This binding to APRIL and BAFF has been shown to stimulate IgM production in peripheral B cells and increase the survival of cultured B cells (3, 4). This data suggests that BCMA may play an important role in B cell development, function and regulation (5). BCMA expression has been found in immune organs and mature B cell lines (5). Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment (6). Within the ECD, cynomolgus monkey BCMA shares 89% sequence identity with human BCMA, 62% with mouse BCMA, and 59% with rat BCMA. The expression of BCMA has also been linked to various cancers, autoimmune disorders, and infectious diseases (7). Proteolytic shedding of the BCMA extracellular domain generated soluble BCMA (sBCMA) via direct cleavage by gamma -secretase, elevated sBCMA levels in serum may correlate with disease activity (8). More recently, BCMA has been indicated as a possible biomarker in various human immunological disease, and as a potential therapeutic target for multiple myeloma (MM) (9-11).
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