Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF

Catalog # Availability Size / Price Qty
10029-BC-050
Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF Bioactivity
2 Images
Product Details
FAQs
Reviews

Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747. The ED 50 for this effect is 30-180 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived BCMA/TNFRSF17 protein
Cynomolgus Monkey BCMA (Met1-Ile54) Accession # XP_005591343 IEGRMD Human IgG 1 (Pro100-Lys330) N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met1
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
33 kDa
SDS-PAGE
37-43 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10029-BC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
    12 months from date of receipt, ≤ -20 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, ≤ -20 degreesC under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Chimera(Catalog # 10029-BC) inhibits Recombinant Human APRIL/TNFSF13 (Catalog # 5860-AP) mediated proliferation of anti-IgM stimulated mouse B cells. The ED50 for this effectis 30-180 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey BCMA/TNFRSF17 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 37‑43 kDa and 75‑85 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, also known as Tumor Necrosis Factor Receptor Superfamily member 17 (gene name TNSFR17), is a member of the TNFR superfamily, due to the presence of its TNFR motif (1). BCMA is a type III membrane protein containing one extracellular cysteine rich domain, a transmembrane domain, and an intracellular domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily (2, 3). This binding to APRIL and BAFF has been shown to stimulate IgM production in peripheral B cells and increase the survival of cultured B cells (3, 4). This data suggests that BCMA may play an important role in B cell development, function and regulation (5). BCMA expression has been found in immune organs and mature B cell lines (5). Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment (6). Within the ECD, cynomolgus monkey BCMA shares 89% sequence identity with human BCMA, 62% with mouse BCMA, and 59% with rat BCMA. The expression of BCMA has also been linked to various cancers, autoimmune disorders, and infectious diseases (7). Proteolytic shedding of the BCMA extracellular domain generated soluble BCMA (sBCMA) via direct cleavage by gamma -secretase, elevated sBCMA levels in serum may correlate with disease activity (8). More recently, BCMA has been indicated as a possible biomarker in various human immunological disease, and as a potential therapeutic target for multiple myeloma (MM) (9-11).

References
  1. Hatzoglou, A. et al. (2000). J Immunol. 165:1322.
  2. Schiemann, B. et al. (2001). Science. 293:2111.
  3. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  4. Huang, H. et al. (2013). PNAS. 110:10928.
  5. Laâbi, Y. et al. (1994). Nucleic Acids Res. 22:1147.
  6. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  7. Coquery, C.M. et al. (2012) Crit Rev Immunol. 32:287.
  8. Laurent, S.A. et al. (2015) Nat Commun. 6:7333.
  9. Thaler, F.S. et al. (2017) Neuro Oncol. 19:1618.
  10. Seckinger, A. et al. (2017) Cancer Cell. 31:396.
  11. Lee, L. et al. (2018) Blood. 131:746.
Long Name
B Cell Maturation Factor
Entrez Gene IDs
608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
Alternate Names
B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF17; tumor necrosis factor receptor superfamily, member 17

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF and earn rewards!

Have you used Recombinant Cynomolgus/Rhesus Macaque BCMA Fc Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review