Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF

His-tag
Catalog # Availability Size / Price Qty
9996-N4-050
Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF Bioactivity
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Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-1 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived Nectin-4 protein
Gly32-Ser349, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gly32
Predicted Molecular Mass
35 kDa
SDS-PAGE
38-45 kDa, reducing conditions

Product Datasheets

9996-N4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF Bioactivity View Larger

Immobilized Recombinant Cynomolgus Monkey Nectin‑4(Catalog # 9996-N4) supports the adhesion of NIH‑3T3 mouse embryonic fibroblastcells. The ED50 for this effect is 0.1-1 μg/mL.

SDS-PAGE Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey Nectin‑4 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 38-45 kDa.

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Background: Nectin-4

Nectin-4, also known as Poliovirus receptor-related protein 4 (Gene name: PVRL4), is a 66-kDa type I transmembrane glycoprotein belonging to the Nectin immunoglobulin superfamily (1). The Latin word necto means "to connect", indicating the role of nectins in Ca2+‑independent cell-cell adhesion (2). Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4 (1‑3). Human Nectin-4 is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain (1, 4, 5). In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is up-regulated and a soluble 43-kDa form is found in the plasma (4-6). This form is generated from the membrane protein via the action of TACE/ADAM-17 (6). Nectin extracellular domains (ECDs) contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type (1, 3). Within the ECD, cynomolgus Nectin-4 shares 99%, and 91% amino acid sequence identity with human and mouse Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins (1, 2). These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections (2, 7). In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development (7). High Nectin-4/PVRL4 expression was associated with poor-prognosis in some invasive breast cancers and may be a new promising prognostic biomarker and specific therapeutic target (8).

References
  1. Reymond, N. et al. (2001) J. Biol. Chem. 276:43205.
  2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
  4. Fabre-Lafay, S. et al. (2007) BMC Cancer 7:73.
  5. Takano, A. et al. (2009) Cancer Res. 69:6694.
  6. Fabre-Lafay, S. et al. (2005) J. Biol. Chem. 280:19543.
  7. Brancati, F. et al. (2010) Am. J. Hum. Genet. 87:265.
  8. M-Rabet, M. et al. (2017) Ann. Oncol. 28:769.
Long Name
Poliovirus Receptor Related 4
Entrez Gene IDs
81607 (Human); 71740 (Mouse); 498281 (Rat); 102127936 (Cynomolgus Monkey)
Alternate Names
EDSS1; Ig superfamily receptor LNIR; LNIR; LNIRnectin 4; Nectin4; Nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PRR4DKFZp686K05193; PVRL4

FAQs

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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