Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF Summary
Gly32-Ser349, with a C-terminal 6-His tag
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
Immobilized Recombinant Cynomolgus Monkey Nectin‑4(Catalog # 9996-N4) supports the adhesion of NIH‑3T3 mouse embryonic fibroblastcells. The ED50 for this effect is 0.1-1 μg/mL.
2 μg/lane of Recombinant Cynomolgus Monkey Nectin‑4 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 38-45 kDa.
Nectin-4, also known as Poliovirus receptor-related protein 4 (Gene name: PVRL4), is a 66-kDa type I transmembrane glycoprotein belonging to the Nectin immunoglobulin superfamily (1). The Latin word necto means "to connect", indicating the role of nectins in Ca2+‑independent cell-cell adhesion (2). Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4 (1‑3). Human Nectin-4 is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain (1, 4, 5). In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is up-regulated and a soluble 43-kDa form is found in the plasma (4-6). This form is generated from the membrane protein via the action of TACE/ADAM-17 (6). Nectin extracellular domains (ECDs) contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type (1, 3). Within the ECD, cynomolgus Nectin-4 shares 99%, and 91% amino acid sequence identity with human and mouse Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins (1, 2). These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections (2, 7). In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development (7). High Nectin-4/PVRL4 expression was associated with poor-prognosis in some invasive breast cancers and may be a new promising prognostic biomarker and specific therapeutic target (8).
- Reymond, N. et al. (2001) J. Biol. Chem. 276:43205.
- Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
- Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.
- Fabre-Lafay, S. et al. (2007) BMC Cancer 7:73.
- Takano, A. et al. (2009) Cancer Res. 69:6694.
- Fabre-Lafay, S. et al. (2005) J. Biol. Chem. 280:19543.
- Brancati, F. et al. (2010) Am. J. Hum. Genet. 87:265.
- M-Rabet, M. et al. (2017) Ann. Oncol. 28:769.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Protein Purification Kits
Reviews for Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF and earn rewards!
Have you used Recombinant Cynomolgus/Rhesus Macaque Nectin-4 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image