Recombinant Cynomolgus SIRP gamma/CD172g Fc Protein, CF

Catalog # Availability Size / Price Qty
10265-SG-050
Recombinant Cynomolgus SIRP gamma/CD172g Fc Protein, CF Bioactivity
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Recombinant Cynomolgus SIRP gamma/CD172g Fc Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 1-8 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey SIRP gamma/CD172g protein
Cynomolgus Monkey SIRP gamma /CD172g
(Glu29-His360)
Accession # XP_005568591.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Glu29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
63 kDa
SDS-PAGE
82-90 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10265-SG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Cynomolgus Monkey SIRPgamma/CD172g Fc Chimera (Catalog # 10265-SG) inhibits anti-CD3-induced proliferation of human T cells. The ED50 for this effect 1‑8 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey SIRP gamma /CD172g Fc Chimera (Catalog # 10265-SG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 82-90 kDa and 170-190 kDa, respectively.

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Reconstitution Calculator

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Background: SIRP gamma/CD172g

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1-5). SIRP members are "paired receptors" with homology in the extracellular domain but variability in the C‑terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N‑glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig‑like domain that contains a J‑like sequence and two C1-type Ig‑like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal C‑type Ig-like domains have been described (5). Within the ECD, cynomolgus monkey SIRP gamma isoform 1 shares 91% aa identity with human SIRP gamma (2).SIRP gamma is the only SIRP known to be expressed on T cells, CD56bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It binds to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells (5, 6). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling motif (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and costimulate T cell activation (5). Engagement of CD47 by SIRP gamma was shown to induce apoptosis on T cell and monocyte cell lines (6).

References
  1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
  2. van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
  3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
  4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
  5. Piccio, L. et al. (2005) Blood 105:2421.
  6. Brooke, G. et al. (2004) J. Immunol. 173:2562.
Long Name
Signal-regulatory Protein gamma
Entrez Gene IDs
55423 (Human); 102138723 (Cynomolgus Monkey)
Alternate Names
bA77C3.1; CD172g antigen; CD172g; FLJ42230; signal-regulatory protein beta 2; Signal-regulatory protein beta-2; signal-regulatory protein gamma; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPB2CD172 antigen-like family member B; SIRP-beta-2; SIRPG; SIRPgamma; SIRP-gamma

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