Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9408-B7-100
Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera Protein, CF Data
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Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.6-3 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey VISTA/B7-H5/PD-1H protein
Cynomolgus Monkey VISTA/B7-H5
(Phe33-Ala194)
Accession # XP_005565644
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Phe33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa
SDS-PAGE
62-71 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9408-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera (Catalog # 9408-B7) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50for this effect is 0.6-3 µg/mL.

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Background: VISTA/B7-H5/PD-1H

V-domain Ig suppressor of T cell activation (VISTA), also known as platelet receptor Gi24, Dies1, SISP1, PD-1H, and B7‑H5, is a 45-55 kDa transmembrane glycoprotein with homology to B7-like immune co-stimulatory molecules (1, 2). Mature cynomologus VISTA contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, cynomologus VISTA shares 96% and 69% aa sequence identity with human and mouse VISTA, respectively. The 30 kDa ECD can be shed by MT1‑MMP, with a 25-30 kDa fragment remaining in the membrane (3). VISTA promotes both MT1‑MMP expression and the MT1-MMP mediated activation of MMP-2 (3). VISTA supports the differentiation of embryonic stem cells (ESC) and enhances BMP4 induced signaling in ESC, but is also down regulated following BMP4 exposure (4, 5). It binds to BMP4 directly, and also associates with the type I BMP receptor Activin RIB/ALK 4 (4, 5). VISTA is highly expressed on mature CD11b high myeloid-derived APCs and to a lesser extent on CD4+, CD8+, and T regs and is also found on tumor infiltrating lymphocytes (7). It is up regulated in vivo on activated monocytes and dendritic cells (5). VISTA inhibits CD4+ and CD8+ T cell proliferation, and their production of IL2 and IFN-gamma (6). Its expression on tumor cells attenuates the antitumor immune response and enables more rapid tumor progression (6). In contrast, VISTA limits disease progression in the autoimmune disease model EAE (6). VISTA-Ig suppressed proliferation of T cells but not B cells and blunted the production of T cell cytokines and activation markers, suggesting that VISTA as a negative checkpoint regulator suppresses T cell activation (8, 9).

References
  1. Flajnik, M.F. et al. (2012) Immunogenetics 64:571.
  2. Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
  3. Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
  4. Aloia, L. et al. (2010) J. Biol. Chem.285:7776.
  5. Parisi, S. et al. (2012) FASEB J. 26:3957.
  6. Wang, L. et al. (2011) J. Exp. Med. 208:577.
  7. Flies, D.B. et al (2014) J Clin Invest. 124:1966.
  8. Lines, J.L. et al. (2014) Cancer Res. 74:1924.
  9. Nowak, E.C. et al. (2017) Immunol Rev. 276:66.
Entrez Gene IDs
64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)
Alternate Names
4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR

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