Recombinant His6-SARS Virus Papain-like Protease Protein, CF

R&D Systems | Catalog # E-610

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Key Product Details

  • R&D Systems E. coli-derived Recombinant His6-SARS Virus Papain-like Protease Protein (E-610)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P0C6U8.1

Applications

Bioactivity
View all Papain-like Protease Proteins and Enzymes »

Product Specifications

Source

E. coli-derived viral Papain-like Protease protein
Glu1541 - Lys1855 with a N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

37 kDa

Activity

Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate.  

Formulation, Preparation, and Storage

E-610
Formulation Supplied as a solution in HEPES, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Papain-like Protease

The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity.  In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.  PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro.  When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species.  

References

  1. Frieman M., et al.  (2009) J.Virol.  83: 6689.
  2. Lindner H.A.,et al.  (2007) Archv. Biochem. Biophys.  466: 8.
  3. Ratia K., et al. (2014) PLoS Pathog. 10:e1004113.
  4. Russell N., et al. (2016) FASEB Ubiquitin & Cell Reg.  (poster)
 

Alternate Names

PL-PRO, PLpro, pp1a, Replicase polyprotein 1a

UniProt

P0C6U8.1

Additional Papain-like Protease Products

Product Documents for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

For research use only

Citations for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

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FAQs for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

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  • Q: Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant His6-SARS Virus Papain-like Protease Protein, CF (Catalog # E-610)?

    A: Recombinant His6-SARS Virus Papain-like Protease Protein, CF (Catalog # E-610) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for catalog # E-610 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).

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