Recombinant His6-SARS Virus Papain-like Protease Protein, CF Summary
MHHHHHHGENLYFQSG, Glu1541 - Lys1855
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Papain-like Protease
The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons. PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro. When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species. This protein contains an N-terminal 6-His tag.
- Frieman M., et al. (2009) J.Virol. 83: 6689
- Lindner H.A.,et al. (2007) Archv. Biochem. Biophys. 466: 8
- Ratia K., et al. (2014) PLoS Pathog doi: 10.1371/journal.ppat.1004113
- Russell N., et al. (2016) FASEB Ubiquitin & Cell Reg. (poster)
Citation for Recombinant His6-SARS Virus Papain-like Protease Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery
Authors: P Tonzi, Y Yin, CWT Lee, E Rothenberg, TT Huang
Sample Types: Protein
Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant His6-SARS Virus Papain-like Protease Protein, CF (Catalog # E-610)?
Recombinant His6-SARS Virus Papain-like Protease Protein, CF (Catalog # E-610) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for catalog # E-610 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).
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