Recombinant His6-SARS Virus Papain-like Protease Protein, CF

Catalog # Availability Size / Price Qty
E-610-050
Product Details
Citations (1)
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Recombinant His6-SARS Virus Papain-like Protease Protein, CF Summary

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Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant SARS virus PLPro is a Ubiquitin- and ISG15-deconjugating enzyme.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate.  Using tetra-Ubiquitin chains as a substrate, PLPro demonstrates a preference for K48 linkages when using a 100 nM enzyme concentration--at higher enzyme concentration other linkages are cleaved as well.
Source
E. coli-derived viral SARS Virus Papain-like Protease protein
MHHHHHHGENLYFQSG, Glu1541 - Lys1855
Accession #
Predicted Molecular Mass
37 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-610

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: Papain-like Protease

The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity.  In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.  PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro.  When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species.  This protein contains an N-terminal 6-His tag.

References
  1. Frieman M., et al.  (2009) J.Virol.  83: 6689
  2. Lindner H.A.,et al.  (2007) Archv. Biochem. Biophys.  466: 8
  3. Ratia K., et al.  (2014) PLoS Pathog  doi: 10.1371/journal.ppat.1004113
  4. Russell N., et al. (2016) FASEB Ubiquitin & Cell Reg.  (poster)
 
Alternate Names
Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a

Citation for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery
    Authors: P Tonzi, Y Yin, CWT Lee, E Rothenberg, TT Huang
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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