Recombinant Human Active Caspase-2 Protein, CF

Catalog # Availability Size / Price Qty

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (3)

Recombinant Human Active Caspase-2 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave the fluorogenic peptide substrate Ac-VDVAD-AFC. The specific activity is >1000 pmol/min/µg, as measured under the described conditions.
E. coli-derived human Caspase-2 protein
Gly170-Asp333 & Ala348-Tyr452
Accession #
N-terminal Sequence
Gly170 & Ala348
Predicted Molecular Mass
12 kDa & 18 kDa
10 kDa & 17 kDa, reducing conditions

Complete Your Research

Product Datasheets

You must select a language.


702-C2/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Sucrose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 25 mM HEPES, 0.1% (w/v) CHAPS, 10 mM DTT, pH 7.5
  • Recombinant Human Active Caspase‑2 (rhCaspase-2) (Catalog # 702-C2/CF)
  • Substrate: Ac-Val-Asp-Val-Ala-Asp-AFC (MP Biomedicals, Catalog # AFC142), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhCaspase-2 to 1 ng/µL in Assay Buffer.
  2. Incubate at room temperature for 15 minutes.
  3. Dilute Substrate to 200 µM with Assay Buffer.
  4. Load into plate 50 µL of 1 ng/µL rhCaspase-2 and start the reaction by adding 50 µL of 200 µM Substrate.
  5. Read at excitation and emission wavelengths of 400 nm and 505 nm (top read), respectively in kinetic mode for 5 minutes.
  6. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-(trifluoromethyl)coumarin (Calbiochem, Catalog # 164580).

Per Well:
  • rhCaspase-2: 0.050 µg
  • Substrate: 100 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Caspase-2

Caspase-2 (Cysteine-aspartic acid protease 2/Casp2; also NEDD2 and ICH-1) is a 30‑32 kDa member of the peptidase C14A/IL‑1 beta ‑converting family of enzymes (1‑3). It is widely expressed and is an integral component of the apoptotic cascade. Based on the length of its prodomain, caspase-2 has been considered to be an initiator caspase. However, studies have shown that other caspases (such as Casp 3) activate procaspase 2, and Caspase-2 likely acts on key cellular molecules such as BID, Golgin 160 and DFF45/ICAD (2, 4, 5). Thus, Caspase-2 is perhaps more likely to be a specialized executioner caspase. Human procaspase-2 is a 48‑51 kDa, 452 amino acid (aa) protein (4‑7). It is known to exist as a disulfide-linked homodimer via covalent linkage at Cys436 (2, 5). But this dimeric state may not be sufficient for (auto)activation. Actual activation may occur following oligomerization within the context of activating platforms such as DISC (death-inducing signaling complex) or the PIDDosome (8‑10). Initially, procaspase-2 undergoes proteolytic cleavage to generate an N-terminal 333 aa p34/34 kDa subunit, and a 119 aa C-terminal p14/14 kDa subunit (5). The p34 and p14 subunits are further processed to generate the prodomain (aa 1‑169), plus the mature p18 (aa 170‑333) and p12 (aa 348‑452) subunits (4‑6). Notably, each p18:p12 noncovalent heterodimer demonstrates proteolytic activity around a catalytic site at aa 318‑322, and, due to an nuclear localization signal within the prodomain, may be found in either nucleus or cytoplasm (11, 12). There are multiple potential isoform variants. Individually, or in combination, there is an alternative start site at Met18, a substitution of two aa for aa 107‑452, a second substitution of 14 aa for aa 309‑322, and a third substitution of 22 aa for aa 323‑452 (6, 7, 13). The human and mouse procaspase 2 precursors are 90% aa identical, with the majority of differences lying in the prodomain.

  1. Chowdhury, I. et al. (2008) Comp. Biochem. Physiol. B 151:10.
  2. Krumschnabel, G. et al. (2009) Cell Death Differ.16:195.
  3. Kitevska, T. et al. (2009) Apoptosis 14:829.
  4. Paroni, G. et al. (2001) J. Biol. Chem. 276:21907.
  5. Li, H. et al. (1997) J. Biol. Chem. 272:21010.
  6. SwissProt. Accession # P42575.
  7. Wang, L. et al. (1994) Cell 78:739.
  8. Chang, D.W. et al. (2003) J. Biol. Chem. 278:16466.
  9. Olsson, M. et al. (2009) Oncogene 28:1949.
  10. Tinel, A. & J. Tschopp (2004) Science 304:843.
  11. Schweizer, A. et al. (2003) J. Biol. Chem. 278:42441.
  12. Colussi, P.A. et al. (1998) J. Biol. Chem. 273:24535.
  13. Droin, N. et al. (2000) Cancer Res. 60:7039.
Entrez Gene IDs
835 (Human); 12366 (Mouse)
Alternate Names
caspase-2; CASP2; CASP-2; caspase 2, apoptosis-related cysteine peptidase; Caspase2; Caspase-2; ICH1; ICH-1; NEDD2; NEDD-2; PPP1R57

Citations for Recombinant Human Active Caspase-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Anamorsin, a novel caspase-3 substrate in neurodegeneration.
    Authors: Yun N, Lee Y, Kim C, Shibayama H, Tanimura A, Hamanaka Y, Kanakura Y, Park I, Jo A, Shin J, Ju C, Kim W, Oh Y
    J Biol Chem, 2014;289(32):22183-95.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  2. Degradomics reveals that cleavage specificity profiles of caspase-2 and effector caspases are alike.
    Authors: Wejda M, Impens F, Takahashi N, Van Damme P, Gevaert K, Vandenabeele P
    J Biol Chem, 2012;287(41):33983-95.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Enzyme Assay
  3. Fine-tuning nucleophosmin in macrophage differentiation and activation.
    Authors: Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, Jacquel A, Cally R, Manoury B, Vanden Berghe T, Vandenabeele P, Droin N, Solary E
    Blood, 2011;118(17):4694-704.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Active Caspase-2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Active Caspase-2 Protein, CF and earn rewards!

Have you used Recombinant Human Active Caspase-2 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review