Activins and inhibins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated or inhibited, respectively, pituitary follicle stimulating hormone (FSH) release. These proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Activins/inhibins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A ( beta A - beta A), activin B ( beta B - beta B), activin AB ( beta A ‑ beta B), inhibin A ( alpha - beta A) and inhibin B ( alpha - beta B). At present, little is known about the contribution of the other beta subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human beta B subunit is greater than 98% identical to mouse beta B, while the human and mouse alpha subunits share approximately 80% identity. Similarly to other TGF-beta family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated as type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling. Besides activins, Act RII has been shown to bind certain other TGF-beta superfamily members. Inhibin A has been shown to bind with low‑affinity to Act RII. The existence of a distinct inhibin-specific receptor and/or signal transduction pathway has been hypothesized.
Recombinant Human Activin B Protein
R&D Systems | Catalog # 659-AB
Key Product Details
Source
CHO
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Activin B protein
Gly293-Ala407
Gly293-Ala407
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly293
Predicted Molecular Mass
12.8 kDa (monomer)
SDS-PAGE
12.4 kDa, reducing conditions
Activity
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
The ED50 for this effect is 0.3‑1.5 ng/mL.
The ED50 for this effect is 0.3‑1.5 ng/mL.
Reviewed Applications
Read 2 reviews rated 3.5 using 659-AB in the following applications:
Formulation, Preparation, and Storage
659-AB
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: Activin B
References
- Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
- Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.
Long Name
Inhibin Subunit Beta B
Alternate Names
activin AB beta polypeptide, Activin Beta B, Activin beta-B chain, INHBB, Inhibin Beta B, inhibin beta B chain, Inhibin Subunit Beta B, inhibin, beta B, inhibin, beta B (activin AB beta polypeptide), Inhibin, beta-2
Gene Symbol
INHBB
UniProt
Additional Activin B Products
Product Documents for Recombinant Human Activin B Protein
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Activin B Protein
For research use only
Related Research Areas
Citations for Recombinant Human Activin B Protein
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Customer Reviews for Recombinant Human Activin B Protein (2)
3.5 out of 5
2 Customer Ratings
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Application: Cell ProliferationVerified Customer | Posted 10/09/2020
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