Recombinant Human Activin RIB/ALK-4 Fc Chimera Protein, CF Summary
|Human Activin RIB
(Leu32 - Glu126)
Accession # AAA60556
(Pro100 - Lys330)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Activin RIB/ALK-4
Activin RIB, also known as ALK4, is a glycosylated 58 kDa type I receptor in the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with and phosphorylates the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature human Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, human Activin RIB shares 93% and 95% aa sequence identity with mouse and rat Activin RIB, respectively. It shares 25% - 35% aa sequence identity with other human type I receptors Activin RIA, Activin RIC, BMPR-IA, BMPR-IB, and TGF-beta R1. Alternately spliced isoforms of Activin RIB have deletions in the cytoplasmic domain and function as dominant negative inhibitors of activin signaling (5, 6). Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (7 - 9). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (10). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation and apoptosis, and tumorigenesis (1, 11, 12).
- Chen, Y.- G. et al. (2006) Exp. Biol. Med. 231:534.
- Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
- Tsuchida, K. et al. (1995) Endocrinology 136:5493.
- ten Dijke, P. et al. (1993) Oncogene 8:2879.
- Xu, J. et al. (1994) Proc. Natl. Acad. Sci. 91:7957.
- Zhou, Y. et al. (2000) Mol. Endocrinol. 14:2066.
- Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
- Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
- Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
- Lewis, K.A. et al. (2000) Nature 404:411.
- Gu, Z. et al. (1998) Genes Dev. 12:844.
- Chen, Y. et al. (2004) Dev. Biol. 268:280.
Citations for Recombinant Human Activin RIB/ALK-4 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
Filter your results:
Functionally diverse heteromeric traps for ligands of the transforming growth factor-beta superfamily
Authors: R Kumar, AV Grinberg, H Li, TH Kuo, D Sako, L Krishnan, K Liharska, J Li, R Grenha, MC Maguire, SD Briscoe, RS Pearsall, BR Herrin, RNVS Suragani, R Castonguay
Scientific Reports, 2021;11(1):18341.
Sample Types: Recombinant Proteins
Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
J. Biol. Chem., 2011;286(34):30034-46.
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human Activin RIB/ALK-4 Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Activin RIB/ALK-4 Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Human Activin RIB/ALK-4 Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image