Recombinant Human Activin RIIA Fc Chimera (CHO) Protein, CF Summary
Approximately 0.01-0.05 μg/mL of Recombinant Human (rh) Activin RIIA Fc Chimera will inhibit 50% of the biological response due to 3 ng/mL of rhActivin A.
|Human Activin RIIA
(Met1 - Pro134)
Accession # P27037
(Pro100 - Lys330)
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Activin RIIA
Activin proteins are involved in a wide range of biological processes including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, the regulation of reproductive physiology, inflammation, and carcinogenesis (1 ‑ 3). They function through heteromeric complexes of type I and type II serine/threonine kinase receptors (2, 4). Dimeric ligands bind to a type II receptor, such as Activin Receptor IIA (ActRIIA), which then associates with a type I receptor to initiate signal transduction (4). ActRIIA mediates the pleiotropic effects of Activins and Inhibins as well as several members of the BMP and GDF families of TGF-beta like proteins (4). Mature human ActRIIA is a 70 kDa glycoprotein that consists of a 116 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 352 aa cytoplasmic region that includes the kinase domain and a PDZ-binding motif (5). Within the ECD, human ActRIIA shares 98% aa sequence identity with mouse and rat ActRIIA. Signaling through ActRIIA is modulated by its interaction with RGM-B/DRAGON, Cripto, Endoglin/CD105, TGF‑ beta RIII/Betaglycan, or BAMBI (6 - 10). These interactions can enhance ligand‑induced signaling or interfere with signaling by preventing ActRIIA association with type I receptors (6 - 9). Activin‑induced responses can also be limited by the enhanced internalization of ActRIIA following its association with the cytoplasmic protein ARIP2 (11).
- Welt, C. et al. (2002) Exp. Biol. Med. 227:724.
- Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
- Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
- de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
- Matzuk, M.M. and A. Bradley (1992) Biochim. Biophys Acta 1130:105.
- Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
- Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
- Lewis, K.A. et al. (2000) Nature 404:411.
- Onichtchouk, D. et al. (1999) Nature 401:480.
- Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
- Matsuzaki, T. et al. (2002) J. Biol. Chem. 277:19008.
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