Recombinant Human Activin RIIA Fc Chimera Protein

Carrier Free

Catalog # Availability Size / Price Qty
340-R2-100/CF

With Carrier

Catalog # Availability Size / Price Qty
340-R2-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (5)
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Recombinant Human Activin RIIA Fc Chimera Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Activin A-induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. Approximately 0.03-0.1 µg/mL of rhActivin RIIA/Fc Chimera will inhibit 50% of the biological response due to 3 ng/mL of rhActivin A.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Activin RIIA protein
Human Activin RIIA
(Ser25 - Pro134)
Accession # P27037
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa (monomer)
SDS-PAGE
50 kDa, reducing conditions

Product Datasheets

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340-R2 (with carrier)

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340-R2/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

340-R2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

340-R2/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Activin RIIA

Activin proteins are involved in a wide range of biological processes including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, the regulation of reproductive physiology, inflammation, and carcinogenesis (1 ‑ 3). They function through heteromeric complexes of type I and type II serine/threonine kinase receptors (2, 4). Dimeric ligands bind to a type II receptor, such as Activin Receptor IIA (ActRIIA), which then associates with a type I receptor to initiate signal transduction (4). ActRIIA mediates the pleiotropic effects of Activins and Inhibins as well as several members of the BMP and GDF families of TGF-beta like proteins (4). Mature human ActRIIA is a 70 kDa glycoprotein that consists of a 116 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 352 aa cytoplasmic region that includes the kinase domain and a PDZ-binding motif (5). Within the ECD, human ActRIIA shares 98% aa sequence identity with mouse and rat ActRIIA. Signaling through ActRIIA is modulated by its interaction with RGM-B/DRAGON, Cripto, Endoglin/CD105, TGF-beta RIII/Betaglycan, or BAMBI (6 - 10). These interactions can enhance ligand-induced signaling or interfere with signaling by preventing ActRIIA association with type I receptors (6 - 9). Activin-induced responses can also be limited by the enhanced internalization of ActRIIA following its association with the cytoplasmic proteins ARIP1 and ARIP2 (11, 12).

References
  1. Welt, C. et al. (2002) Exp. Biol. Med. 227:724.
  2. Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
  3. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
  4. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  5. Matzuk, M.M. and A. Bradley (1992) Biochim. Biophys Acta 1130:105.
  6. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
  8. Lewis, K.A. et al. (2000) Nature 404:411.
  9. Onichtchouk, D. et al. (1999) Nature 401:480.
  10. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  11. Shoji, H. et al. (2000) J. Biol. Chem. 275:5485.
  12. Matsuzaki, T. et al. (2002) J. Biol. Chem. 277:19008.
    Long Name
    Activin Receptor IIA
    Entrez Gene IDs
    92 (Human); 11480 (Mouse)
    Alternate Names
    activin A receptor, type II; activin A receptor, type IIA; Activin receptor type IIA; activin receptor type-2A; Activin RIIA; ActivinRIIA; ACTRII; ACTRIIA; ACVR2A; ACVR2ACTR-IIA; AVR2A; EC 2.7.11; EC 2.7.11.30

    Citations for Recombinant Human Activin RIIA Fc Chimera Protein

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    5 Citations: Showing 1 - 5
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    1. Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology
      Authors: K Sakamoto, Y Kanematsu-, Y Kamada, M Oka, T Ohnishi, M Miwa, T Asami, H Inooka
      Biochem Biophys Rep, 2017-06-10;11(0):33-39.
      Applications: Bioassay
    2. Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis
      Authors: BR Yoon, YH Chung, SJ Yoo, K Kawara, J Kim, IS Yoo, CG Park, SW Kang, WW Lee
      J. Biol. Chem, 2015-12-23;291(8):4048-57.
      Species: Human
      Sample Types:
      Applications: Bioassay
    3. Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains
      Authors: Y Kienast, U Jucknischk, S Scheiblich, M Thier, M de Wouters, A Haas, C Lehmann, V Brand, D Bernicke, K Honold, S Lorenz
      J. Biol. Chem, 2015-12-16;291(7):3395-410.
      Species: Human
      Sample Types:
      Applications: Bioassay
    4. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
      Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
      J. Mol. Biol., 2008-07-02;381(4):1025-39.
      Species: Human
      Sample Types: Recombinant Protein
      Applications: ELISA (detection), Surface Plasmon Resonance
    5. Transforming growth factor beta1 regulates follistatin mRNA expression during in vitro bovine granulosa cell differentiation.
      Authors: Fazzini M, Vallejo G, Colman-Lerner A, Trigo R, Campo S, Baranao JL, Saragueta PE
      J. Cell. Physiol., 2006-04-01;207(1):40-8.
      Species: Bovine
      Sample Types: Whole Cells
      Applications: Bioassay

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