Recombinant Human ADAM23 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Human ADAM23 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is 1.0‑4.0 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human ADAM23 protein
Ser60-His585 with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ser60 (pro) & Ala287 (mature)
Predicted Molecular Mass
60.8 kDa (pro) & 34.9 kDa (mature)
40‑60 kDa, reducing conditions

Complete Your Experiment

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: ADAM23

ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 100 kDa glycosylated proprotein that consists of a 227 amino acid (aa) propeptide, a 506 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 19 aa cytoplasmic domain (1, 2). The ECD contains a nonfunctional metalloprotease domain, an integrin‑binding disintegrin domain, and a cysteine‑rich region. Proteolytic removal of the propeptide generates an approximately 70 kDa mature protein (2). Within the propeptide, peptidase, and disintegrin domains (aa 60‑585), human ADAM23 shares 93% aa sequence identity with mouse and rat ADAM23. Alternative splicing of human ADAM23 yields additional isoforms (including a potentially secreted molecule) by substitution of the transmembrane and cytoplasmic regions (3). ADAM23 is expressed in the brain, notably on pyramidal cells of the cerebral cortex and hippocampus and on cerebellar Purkinje and granule cells (1, 2, 4‑6). ADAM23 binds the prion protein PrPC and the leucine‑rich glioma inactivated proteins LGI1 and LGI4 (7, 8). Its interaction with LGI1 promotes neurite outgrowth and dendrite arborization on pyramidal neurons (5). LGI1 appears to function as a trans‑synaptic bridge between ADAM23 and ADAM22 (6). In adipose tissue, the binding of ADAM23 to LGI3 inhibits adipocyte differentiation and lipid accumulation (9). ADAM23 is also expressed on bronchial epithelial cells (10). It serves as a counter‑receptor for Integrin alpha V beta 3 and can limit integrin activation and adhesion to alpha V beta 3 ligands (4, 11). ADAM23 expression is down‑regulated in some breast and non‑small cell lung carcinomas (10, 12).

  1. Sagane, K. et al. (1998) Biochem. J. 334:93.
  2. Goldsmith, A.P. et al. (2004) J. Neurosci. Res. 78:647.
  3. Sun, Y.P. et al. (2004) Gene 325:171.
  4. Cal, S. et al. (2000) Mol. Biol. Cell. 11:1457.
  5. Owuor, K. et al. (2009) Mol. Cell. Neurosci. 42:448.
  6. Fukata, Y. et al. (2010) Proc. Natl. Acad. Sci. USA 107:3799.
  7. Costa, M.D.M. et al. (2009) Neurosci. Lett. 461:16.
  8. Sagane, K. et al. (2008) Int. J. Biol. Sci. 4:387.
  9. Kim, H.A. et al. (2012) Biochim. Biophys. Acta 1821:914.
  10. Hu, C. et al. (2011) Int. J. Exp. Pathol. 92:333.
  11. Verbisck, N.V. et al. (2009) Cancer Res. 69:5546.
  12. Costa, F.F. et al. (2004) Oncogene 23:1481.
Long Name
A Disintegrin and Metalloproteinase Domain 23
Entrez Gene IDs
8745 (Human); 23792 (Mouse); 301460 (Rat)
Alternate Names
a disintegrin and metalloproteinase domain 23; ADAM metallopeptidase domain 23; ADAM23; disintegrin and metalloproteinase domain-containing protein 23; MDC3; MDC-3; MDC3ADAM 23; MDC-L; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 3


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human ADAM23 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human ADAM23 Protein, CF and earn rewards!

Have you used Recombinant Human ADAM23 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review