Recombinant Human ADAM28 Protein, CF

R&D Systems | Catalog # 9325-AD

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human ADAM28 Protein (9325-AD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q9UKQ2

Applications

Bioactivity
View all ADAM28 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ADAM28 protein
Leu72 & Leu191-Ala623, with a C-terminal 6-His tag

Purity

>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu72 & Leu191

Predicted Molecular Mass

49 & 62 kDa

SDS-PAGE

59-80 kDa, reducing conditions

Activity

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
The ED50 for this effect is 0.250-2.50 μg/mL.

Reviewed Applications

Read 2 reviews rated 5 using 9325-AD in the following applications:

Scientific Data Images for Recombinant Human ADAM28 Protein, CF

Recombinant Human ADAM28 Protein Bioactivity

Recombinant Human ADAM28 Protein Bioactivity.

Recombinant Human ADAM28 (Catalog # 9325-AD) induces adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.250-2.50 μg/mL.

Formulation, Preparation, and Storage

9325-AD
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution

Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: ADAM28

A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 amino acid (aa) extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3).  ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha 4b1(9). 

References

  1. Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
  2. McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
  3. Howard, L. et al. (2000) Biochem. J. 348:21.
  4. Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
  5. Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
  6. Blobel, C.P. (1997) Cell 90:589.
  7. Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
  8. Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
  9. Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.

Long Name

A Disintegrin and Metalloproteinase Domain 28

Alternate Names

Dtgn1, eMDCII, MDC-Lm, MDC-Ls, MDCL, Protein-L

Entrez Gene IDs

10863 (Human); 13522 (Mouse); 290344 (Rat)

Gene Symbol

ADAM28

UniProt

Q9UKQ2

Additional ADAM28 Products

Product Documents for Recombinant Human ADAM28 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ADAM28 Protein, CF

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Recombinant Human ADAM28 Protein, CF (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Human ADAM28 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Recombinant Human ADAM28 Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/17/2018
    Recombinant Human ADAM28 Protein, CF 9325-AD
  • Recombinant Human ADAM28 Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/16/2018
    Recombinant Human ADAM28 Protein, CF 9325-AD

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes