Recombinant Human ADAMTS4 Protein, CF
Recombinant Human ADAMTS4 Protein, CF Summary
Phe213-Cys685, with a C-terminal 10-His tag
|Formulation||Supplied as a 0.2 μm filtered solution in Sodium Acetate, CaCl2 and NaCl.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Assay Buffer: 50 mM HEPES, 50 mM NaCl, 1 mM CaCl2, 0.05% Brij-35, pH 7.5
- Recombinant Human ADAMTS4 (Catalog # 4307-AD)
- Substrate: WAAG-3R (Anaspec, Catalog # 60431-1), 2 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhADAMTS4 to 10 µg/mL in Assay Buffer.
- Dilute Substrate to 50 µM in Assay Buffer.
- Load 50 µL of 10 µg/mL of rhADAMTS4 into a plate, and start the reaction by adding 50 µL of 50 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
- Read at excitation and emission wavelengths of 340 nm and 420 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
Specific Activity (pmol/min/µg) =
|Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)|
|amount of enzyme (µg)|
*Adjusted for Substrate Blank
**Derived using calibration standard Abz-Gly-OH (Bachem, Catalog # E-2920).
- rhADAMTS4: 0.5 µg
- Substrate: 25 µM
ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1-3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha -2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4-6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified rhADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5 mM 1 10‑phenanthroline and Recombinant Human TIMP‑3 (Catalog # 973-TM).
Citations for Recombinant Human ADAMTS4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Cleavage of Fibulin-2 by the aggrecanases ADAMTS-4 and ADAMTS-5 contributes to the tumorigenic potential of breast cancer cells
Authors: T Fontanil, S ?lvarez-Te, M? Villaronga, Y Mohamedi, L Solares, A Moncada-Pa, JA Vega, O Garc¡a-Su , M P‚rez-Bast, JM Garc¡a-Ped, AJ Obaya, S Cal
Sample Types: Protein
Applications: Enzyme Assay
ADAMTS-4 promotes neurodegeneration in a mouse model of amyotrophic lateral sclerosis.
Authors: Lemarchant S, Pomeshchik Y, Kidin I, Karkkainen V, Valonen P, Lehtonen S, Goldsteins G, Malm T, Kanninen K, Koistinaho J
Mol Neurodegener, 2016;11(1):10.
Sample Types: Whole Cells
ADAMTS4 and its proteolytic fragments differentially affect melanoma growth and angiogenesis in mice.
Authors: Rao N, Ke Z, Liu H, Ho C, Kumar S, Xiang W, Zhu Y, Ge R
Int J Cancer, 2013;133(2):294-306.
Sample Types: N/A
Applications: WB Ctrl
Simple pseudo-dipeptides with a P2' glutamate: a novel inhibitor family of matrix metalloproteases and other metzincins.
Authors: Devel L, Beau F, Amoura M, Vera L, Cassar-Lajeunesse E, Garcia S, Czarny B, Stura E, Dive V
J Biol Chem, 2012;287(32):26647-56.
Sample Types: Fluorogenic Peptide Substrate
The endogenous proteoglycan-degrading enzyme ADAMTS-4 promotes functional recovery after spinal cord injury.
Authors: Tauchi R, Imagama S, Natori T
J Neuroinflammation, 2012;9(0):53.
Sample Types: In Vivo
Applications: In Vivo
Processing of the matricellular protein hevin in mouse brain is dependent on ADAMTS4.
Authors: Weaver MS, Workman G, Cardo-Vila M, Arap W, Pasqualini R, Sage EH
J. Biol. Chem., 2010;285(8):5868-77.
Sample Types: Whole Cells
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