Recombinant Human AG-2/AGR2 His-tag Protein, CF

R&D Systems | Catalog # 10326-AG

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human AG-2/AGR2 His-tag Protein (10326-AG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

NP_006399.1

Structure / Form

Noncovalently-linked homodimer

Applications

Bioactivity
View all AG-2/AGR2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human AG-2/AGR2 protein
Arg21-Leu175, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met-Arg21

Predicted Molecular Mass

19 kDa

SDS-PAGE

17-20 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of PC-3 human prostate cancer cells. 

The ED50 for this effect is 0.3-1.8 µg/mL.

Scientific Data Images for Recombinant Human AG-2/AGR2 His-tag Protein, CF

Recombinant Human AG-2/AGR2 His-tag Protein Bioactivity

Recombinant Human AG-2/AGR2 His-tag Protein Bioactivity

Recombinant Human AG-2/AGR2 His-tag (Catalog # 10326-AG) supports the adhesion of PC-3 human prostate cancer cells. The ED50 for this effect is 0.03-0.18 μg/mL.
Recombinant Human AG-2/AGR2 His-tag Protein SDS-PAGE

Recombinant Human AG-2/AGR2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human AG-2 His-tag (Catalog # 10326-AG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-20 kDa.

Formulation, Preparation, and Storage

10326-AG
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, and TCEP.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AG-2/AGR2

AG-2 (Anterior gradient protein 2; also AGR2, GOB4 and HPC8) is an 18‑21 kDa member of the Protein disulfide-isomerase (PDI) family of enzymes. PDIs participate in the formation of transient disulfide linkages with molecules which aid in protein folding. Mature human AG-2 is 155 amino acids (aa) in length (aa 21‑175) and has a dimeric thioredoxin fold structure (1) which contains one cysteine (Cys81). It is expressed strongly in secretory cell types, such as prostate epithelium, small intestine goblet, trachea and lung, and is also expressed in estrogen receptor-positive breast cancer cell lines (2). Although AG-2 possesses a signal sequence, it appears to be rarely secreted, instead being found in the ER due the presence of a KTEL ER retention signal at its C-terminus (3). Over aa 21‑175, human AG-2 shares 94% aa identity with mouse AG-2. Over expressed in colorectal cancer stem cells, AG-2 is a marker regulated by the canonical Wnt/ beta -catenin pathway, AG-2 levels combined with elevated levels of beta -catenin are shown to indicate lower survival prognosis (4). Further, Wnt11-mediated signaling pathway has been implicated in secreted AG-2's role to promote metastasis of colorectal cancer (5). In prostate cancer, marker RAD9A affects the availability of AG-2. When added to RAD9A-depleted PC-3 cells, addition of AG-2 restores cell migration and adhesion properties (6). Additionally, AG-2 has been linked to pituitary adenomas as a biomarker (7).

References

  1. Patel, P. et al. (2013) ‎J. Mol. Biol. 425:929.
  2. Thompson, D. A. and Weigel, R. J. (1998) Biochem. Biophys. Res. Commun. 251:111.
  3. Gupta, A. et al. (2012) J. Biol. Chem. 287:4773.
  4. Lamichane, B. et al. (2019) Biochem. Biophys. Res. Commun. 515:600.
  5. Tian, S. et al. (2018) Experimental Cell Research. 364:198.
  6. Broustas, C. G. et al. (2018) Carcinogenesis. 40:164.
  7. Tohti, M. et al. (2017) Clinical Neurology and Neurosurgery. 154:19.

Long Name

Anterior Gradient 2 Homolog

Alternate Names

AG2, AGR2, GOB-4, HPC8, PDIA17

Entrez Gene IDs

10551 (Human); 23795 (Mouse); 298961 (Rat)

Gene Symbol

AGR2

UniProt

NP_006399.1

Additional AG-2/AGR2 Products

Product Documents for Recombinant Human AG-2/AGR2 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human AG-2/AGR2 His-tag Protein, CF

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Recombinant Human AG-2/AGR2 His-tag Protein, CF

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