Recombinant Human AICL/CLEC-2B Fc Chimera Protein, CF

  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human AICL/CLEC-2B Fc Chimera is coated at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Galectin-1 (Catalog # 1152-GA) that produces 50% optimal binding response is 0.6-3 µg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    MD Human IgG1
    (Lys26-His149) with a Met79Thr substitution
    Accession # Q92478
    N-terminus C-terminus
  • N-terminal Sequence
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    41 kDa
    48-65 kDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images

When Recombinant HumanAICL/CLEC-2B Fc Chimera (Catalog #
9059-CL) is coated at 2 µg/mL, Recombinant Human Galectin-1(Catalog # 1152-GA) binds with a typical ED50 of
0.6-3 µg/mL.

Background: AICL/CLEC-2B
Activation-induced C-type lectin (AICL), also known as CLEC2B, is a 30-35 kDa variably glycosylated type-2 transmembrane member of the C-type lectin-like receptor (CTLR) family. AICL belongs to the subgroup of CLEC2 proteins that also includes CLEC2A/KACL, CLEC2D/LLT, and CD69/CLEC2C, all of which are encoded by the natural killer gene complex (1). Human AICL contains a single C-type lectin domain in its extracellular region and a 7 amino acid cytoplasmic tail (2). AICL is expressed on monocytes, macrophages, and granulocytes (3), and it is upregulated on TLR-activated monocytes and IL-12 + IL-18 activated NK cells (3, 4). AICL is an activating receptor that triggers TNF production by monocytes (3). It binds to NKp80 on NK cells, resulting in NK cell mediated lysis of the AICL expressing monocyte (3). In addition, the AICL-NKp80 axis mediates interactions between activated and resting NK cells (4).
  • References:
    1. Li, Y. et al. (2014) Front. Immunol. 5:123.
    2. Hamann, J. et al. (1997) Immunogenetics 45:295.
    3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
    4. Klimosch, S.N. et al. (2013) Blood 122:2380.
  • Long Name:
    Activation-Induced C-Type Lectin
  • Entrez Gene IDs:
    9976 (Human)
  • Alternate Names:
    Activation-induced C-type lectin; AICL; AICLC-type lectin superfamily member 2; CLEC2B; CLEC-2B; CLECSF2; CLECSF2IFN-alpha-2b-inducing-related protein 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 2 (activation-induced); C-type lectin domain family 2 member B; C-type lectin domain family 2, member B; HP10085; IFN-alpha2b-inducing related protein 1; IFNRG1
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