Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF

R&D Systems | Catalog # 7678-DH

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein (7678-DH)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P42330

Applications

Enzyme Activity
View all Aldo-keto Reductase 1C3/AKR1C3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Aldo-keto Reductase 1C3/AKR1C3 protein
Asp2-Tyr323, with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

38 kDa

SDS-PAGE

36-37 kDa, reducing conditions

Activity

Measured by its ability to catalyze the reduction of phenanthrenequinone.
The specific activity is >115 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 2 reviews rated 5 using 7678-DH in the following applications:

Formulation, Preparation, and Storage

7678-DH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aldo-keto Reductase 1C3/AKR1C3

AKR1C3 is a member of the aldo-keto reductase (AKR) superfamily. It catalyzes oxidation/reduction reactions at the 3-alpha, 20-alpha, and 17-beta positions of steroids (1). It is also known as Prostaglandin F synthase as it reduces prostaglandin D2 to F2, and therefore may play a role in allergic conditions such as asthma (2). Elevated expression of AKR1C3 in endometrium that results in enhanced estrogen action may lead to endometrial cancer (3). It is also up‑regulated in squamous cell carcinoma of head and neck (4). AKR1C3 is found to be a novel suppressor of cell differentiation that provides a plausible target for the non-cyclooxygenase-dependent antineoplastic actions of nonsteroidal anti-inflammatory drugs (5).

References

  1. Penning, T. M. et al. (2000) Biochem J. 351:67.
  2. Suzuki-Yamamoto, T. et al. (1999) FEBS Lett. 462:335.
  3. Rizner, T. L. et al. (2006) Mol. Cell. Endocrinol. 248:126.
  4. Li, S. et al. (2004) Br. J. Cancer 90:1093.
  5. Desmond, J. C. et al. (2003) Cancer Res. 63:505.

Long Name

Aldo-keto Reductase Family 1, Member C3

Alternate Names

AKR1C3, AldoketoReductase 1C3, DD3, DDX, HA1753, HAKRB, HSD17B5, PGFS

Entrez Gene IDs

8644 (Human); 105349 (Mouse); 171516 (Rat)

Gene Symbol

AKR1C3

UniProt

P42330 (Human)

Additional Aldo-keto Reductase 1C3/AKR1C3 Products

Product Documents for Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Citations for Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF

Customer Reviews for Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF (2)

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    Application: Enzymatic activity in vitro
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    Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF 7678-DH
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    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 10/10/2018
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Protocols

View specific protocols for Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF (7678-DH):

Materials
  • Assay Buffer: 100 mM Sodium Phosphate, pH 6.0
  • Recombinant Human Aldo‑keto Reductase 1C3/AKR1C3 (rhAKR1C3) (Catalog # 7678-DH)
  • 9,10-Phenanthrenequinone (PQ) (Sigma, Catalog # 156507), 5 mM stock in N,N-Dimethylformamide
  • beta -Nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt ( beta -NADPH) (Sigma, Catalog # N7505), 10 mM in deionized water
  • UV Plate (Costar, Catalog # 3635)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhAKR1C3 to 20 μg/mL in Assay Buffer.
  2. Prepare a Reaction Mixture containing 40 μM PQ and 400 μM beta -NADPH in Assay Buffer.
  3. In a plate, load 50 μL of 20 μg/mL rhAKR1C3, and start the reaction by adding 50 μL of Reaction Mixture.  Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of Reaction Mixture.
  4. Read at an absorbance of 340 nm in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x -1 x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 6270 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD Per Well:
  • rhAKR1C3: 1 μg
  • PQ: 20 μM
  • beta -NADPH: 200 μM

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