Recombinant Human ALK-1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
370-AL-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (15)
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Recombinant Human ALK-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit BMP-9-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is 3-12 ng/mL in the presence of 2 ng/mL of rhBMP-9.
Source
Mouse myeloma cell line, NS0-derived human ALK-1 protein
Human ALK-1
(Asp22 - Gln118)
Accession # P37023.2
IEGRID Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
37.3 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions

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370-AL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

370-AL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: ALK-1

Transforming Growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine- and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

References
  1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  2. ten Dijke, P. et al. (1994) Science 264:101.
  3. Lux, A. et al. (1999) J. Biol. Chem. 274:9984.
Long Name
Activin Receptor-like Kinase 1
Entrez Gene IDs
94 (Human); 11482 (Mouse)
Alternate Names
activin A receptor type II-like 1; activin A receptor, type II-like kinase 1; Activin receptor-like kinase 1; ACVRL1; ACVRLK1ORW2; ALK1; ALK-1; ALK1TGF-B superfamily receptor type I; EC 2.7.11; HHT; HHT2EC 2.7.11.30; serine/threonine-protein kinase receptor R3; SKR3; TSR-I

Citations for Recombinant Human ALK-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Atheroprone fluid shear stress-regulated ALK1-Endoglin-SMAD signaling originates from early endosomes
    Authors: PL Mendez, L Obendorf, J Jatzlau, W Burdzinski, M Reichenbac, V Nageswaran, A Haghikia, V Stangl, C Hiepen, P Knaus
    Bmc Biology, 2022;20(1):210.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Crystal structures of BMPRII extracellular domain in binary and ternary receptor complexes with BMP10
    Authors: J Guo, B Liu, M Thorikay, M Yu, X Li, Z Tong, RM Salmon, RJ Read, P Ten Dijke, NW Morrell, W Li
    Nature Communications, 2022;13(1):2395.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Surface Plasmon Resonance
  3. Decreased Colonic Activin Receptor-Like Kinase 1 Disrupts Epithelial Barrier Integrity in Patients with Crohn's disease
    Authors: T Toyonaga, EC Steinbach, BP Keith, JB Barrow, MR Schaner, EA Wolber, C Beasley, J Huling, Y Wang, NL Allbritton, N Chaumont, TS Sadiq, MJ Koruda, A Jain, MD Long, EL Barnes, HH Herfarth, KL Isaacs, JJ Hansen, MT Shanahan, R Rahbar, TS Furey, P Sethupathy, SZ Sheikh
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Homoharringtonine enhances the effect of imatinib on chronic myelogenous leukemia cells by downregulating ZFX
    Authors: J Wu, B Wei, Y Shi, X Lu, Y Ding, C Wang, Y Li
    Mol Med Rep, 2019;20(4):3233-3239.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. TGF-? contamination of purified recombinant GDF15
    Authors: OE Olsen, A Skjærvik, BF Størdal, A Sundan, T Holien
    PLoS ONE, 2017;12(11):e0187349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
    Sci Rep, 2016;5(0):37366.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Genome-wide RNAi screen reveals ALK1 mediates LDL uptake and transcytosis in endothelial cells.
    Authors: Kraehling J, Chidlow J, Rajagopal C, Sugiyama M, Fowler J, Lee M, Zhang X, Ramirez C, Park E, Tao B, Chen K, Kuruvilla L, Larrivee B, Folta-Stogniew E, Ola R, Rotllan N, Zhou W, Nagle M, Herz J, Williams K, Eichmann A, Lee W, Fernandez-Hernando C, Sessa W
    Nat Commun, 2016;7(0):13516.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Serum induces transcription of Hey1 and Hey2 genes by Alk1 but not Notch signaling in endothelial cells.
    Authors: Woltje K, Jabs M, Fischer A
    PLoS ONE, 2015;10(3):e0120547.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
    Authors: van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
    J. Biol. Chem., 2012;287(22):18551-61.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  10. Repulsive guidance molecule (RGM) family proteins exhibit differential binding kinetics for bone morphogenetic proteins (BMPs).
    Authors: Wu, Qifang, Sun, Chia Chi, Lin, Herbert, Babitt, Jodie L
    PLoS ONE, 2012;7(9):e46307.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  11. Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
    Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
    Blood, 2012;119(21):5037-47.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  12. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  13. Antitumor activity of ALK1 in pancreatic carcinoma cells.
    Authors: Ungefroren H, Schniewind B, Groth S, Chen WB, Muerkoster SS, Kalthoff H, Fandrich F
    Int. J. Cancer, 2007;120(8):1641-51.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  14. Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells.
    Authors: David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S
    Blood, 2007;109(5):1953-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Cyclic stretch controls the expression of CD40 in endothelial cells by changing their transforming growth factor-beta1 response.
    Authors: Korff T, Aufgebauer K, Hecker M
    Circulation, 2007;116(20):2288-97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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