Recombinant Human alpha 1B-Glycoprotein Protein, CF

Catalog # Availability Size / Price Qty
7757-GP-050
Product Details
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Recombinant Human alpha 1B-Glycoprotein Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CRISP‑3 (Catalog # 2397-CR) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human  alpha  1B‑Glycoprotein that produces 50% of the optimal binding response is approximately 0.4‑2.4 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human alpha 1B-Glycoprotein protein
Ala22-Ser495, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Starts at Ala22
Structure / Form
Monomer
Predicted Molecular Mass
52.8 kDa
SDS-PAGE
70-80 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7757-GP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha 1B-Glycoprotein

Alpha-1B-glycoprotein (A1BG) is an approximately 80 kDa molecule that circulates in the serum (1). Mature human A1BG consists of five tandem Ig‑like domains and shares 44% amino acid sequence identity with mouse and rat A1BG (2, 3). Alternative splicing generates an isoform that lacks the signal peptide and the first Ig‑like domain. A1BG binds with high affinity to the cysteine‑rich protein CRISP‑3 (4). In pancreatic carcinoma, it is elevated in pancreatic juice as well as in the serum relative to normal patients (5, 6). A1BG is abnormally excreted into the urine during idiopathic nephrotic syndrome (INS) (7). Multiple breakdown fragments of A1BG are also present in INS urine, including one of 14 kDa that is found in steroid‑resistant but not steroid‑sensitive cases (7). A1BG is also elevated in the urine of patients with interstitial cystitis/painful bladder syndrome (IC/PBS) (8).

References
  1. Schultze, H.E. et al. (1963) Nature 200:1103.
  2. Ishioka, N. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2363.
  3. Gardmo, C. et al. (2001) Endocrinology 142:2695.
  4. Udby, L. et al. (2004) Biochemistry 43:12877.
  5. Tian, M. et al. (2008) BMC Cancer 8:241.
  6. Li, C. et al. (2009) J. Proteome Res. 8:483.
  7. Piyaphanee, N. et al. (2011) Proteomics Clin. Appl. 5:334.
  8. Goo, Y.A. et al. (2010) Int. Braz. J. Urol. 36:464.
Entrez Gene IDs
1 (Human); 117586 (Mouse)
Alternate Names
A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; GAB

FAQs

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Recombinant Human alpha 1B-Glycoprotein Protein, CF
By Anonymous on 05/23/2016
Application: Binding assay/Protein-protein interaction