Recombinant Human Aminoacylase/ACY1 Protein, CF

R&D Systems | Catalog # 2900-ZN

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Human Aminoacylase/ACY1 Protein (2900-ZN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q03154

Applications

Enzyme Activity
View all Aminoacylase/ACY1 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Aminoacylase/ACY1 protein
Thr2-Ser408, with a C-terminal 10-His tag
Accession # Q03154

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr2

Predicted Molecular Mass

47 kDa

SDS-PAGE

42 kDa, reducing conditions

Activity

Measured by its ability to cleave N-acetyl-L-Methione (Ac-Met).
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation, and Storage

2900-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aminoacylase/ACY1

The human ACY1 gene encodes Aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active as described in Activity Assay Protocol.

References

  1. Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
  2. Maceyka, M. et al. (2004) FEBS Lett. 568:30.

Alternate Names

ACY1

Entrez Gene IDs

95 (Human); 109652 (Mouse)

Gene Symbol

ACY1

UniProt

Q03154

Additional Aminoacylase/ACY1 Products

Product Documents for Recombinant Human Aminoacylase/ACY1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Aminoacylase/ACY1 Protein, CF

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Recombinant Human Aminoacylase/ACY1 Protein, CF

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Protocols

View specific protocols for Recombinant Human Aminoacylase/ACY1 Protein, CF (2900-ZN):

Materials
  • Assay Buffer: 0.1 M Potassium Phosphate, pH 7.0
  • Recombinant Human Aminoacylase/ACY1 (rhACY1) (Catalog # 2900-ZN)
  • Substrate: N-Ac-Met (Sigma, Catalog # 1310), 1 M in DMSO
  • 96-well Clear UV Plate (Corning, Catalog # 3635)
  • Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute Substrate to 40 mM in Assay Buffer.
  2. Load 90 µL of 40 mM Substrate to wells of the UV plate.
  3. Incubate plate at 25 °C for 10 minutes.
  4. Dilute rhACY1 to 200 µg/mL in Assay Buffer.
  5. Load 10 µL of 200 µg/mL rhACY1 to each well containing 90 µL of Substrate. Include a Substrate Blank with 90 µL of Substrate and 10 µL of Assay Buffer.
  6. Incubate plate at 25 °C for 15 minutes.
  7. Read at a wavelength of 238 nm (bottom read) in endpoint mode.
  8. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard N-Acetyl-L-Methionine (Sigma, Catalog # 1310).

Per Well:
  • rhACY1: 2 µg
  • Substrate N-Ac-Met: 36 mM

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