Recombinant Human Aminoacylase/ACY1 Protein, CF Summary
Thr2-Ser408, with a C-terminal 10-His tag
Accession # Q03154
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in Tris and NaCl.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Assay Buffer: 0.1 M Potassium Phosphate, pH 7.0
- Recombinant Human Aminoacylase/ACY1 (rhACY1) (Catalog # 2900-ZN)
- Substrate: N-Ac-Met (Sigma, Catalog # 1310), 1 M in DMSO
- 96-well Clear UV Plate (Corning, Catalog # 3635)
- Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute Substrate to 40 mM in Assay Buffer.
- Load 90 µL of 40 mM Substrate to wells of the UV plate.
- Incubate plate at 25 °C for 10 minutes.
- Dilute rhACY1 to 200 µg/mL in Assay Buffer.
- Load 10 µL of 200 µg/mL rhACY1 to each well containing 90 µL of Substrate. Include a Substrate Blank with 90 µL of Substrate and 10 µL of Assay Buffer.
- Incubate plate at 25 °C for 15 minutes.
- Read at a wavelength of 238 nm (bottom read) in endpoint mode.
- Calculate specific activity:
Specific Activity (pmol/min/µg) =
|Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)|
|Incubation time (min) x amount of enzyme (µg)|
*Adjusted for Substrate Blank
**Derived using calibration standard N-Acetyl-L-Methionine (Sigma, Catalog # 1310).
- rhACY1: 2 µg
- Substrate N-Ac-Met: 36 mM
The human ACY1 gene encodes Aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active as described in Activity Assay Protocol.
- Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
- Maceyka, M. et al. (2004) FEBS Lett. 568:30.
Citation for Recombinant Human Aminoacylase/ACY1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Serum aminoacylase-1 is a novel biomarker with potential prognostic utility for long-term outcome in patients with delayed graft function following renal transplantation.
Authors: Welberry Smith, Matthew, Zougman, Alexandr, Cairns, David A, Wilson, Michelle, Wind, Tobias, Wood, Steven L, Thompson, Douglas, Messenger, Michael, Mooney, Andrew, Selby, Peter J, Lewington, Andrew J, Banks, Rosamond
Kidney Int, 2013;84(6):1214-25.
Sample Types: Serum
Applications: ELISA (Standard)
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human Aminoacylase/ACY1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Aminoacylase/ACY1 Protein, CF and earn rewards!
Have you used Recombinant Human Aminoacylase/ACY1 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image