Recombinant Human Angiopoietin-1 Protein, CF

(39 citations)   
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al. (1999) FEBS Letters 448:249. The ED50 for this effect is 10-40 ng/mL in the presence of 5 µg/mL of a
    cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
  • Source
    Mouse myeloma cell line, NS0-derived Ser20-Phe498, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser20
  • Structure / Form
    Oligomer
  • Predicted Molecular Mass
    56 kDa
  • SDS-PAGE
    70 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
923-AN/CF
 
923-AN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-1
Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system (1, 2). It contains a coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region (3, 4). Mature human Angiopoietin-1 shares 97% amino acid sequence identity with mouse and rat Angiopoietin-1. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into
disulfide-linked homotrimers, tetramers, and pentamers (3, 5). Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation (3, 4). Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels (6, 7). In sub-confluent EC (i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell (8). In confluent EC (i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the in trans homotypic association of Tie-2 between EC or with the substratum (8, 9). In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin (10-12). Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2 (13, 14). These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes (13, 14). Angiopoietin-1 can protect against pulmonary arterial hypertension (5), reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium (15), and enhance tumor progression and metastasis (16).
  • References:
    1. Koh, G.Y. (2012) Trends Mol. Med. 19:31.
    2. Suri, C. et al. (1996) Cell 87:1171.
    3. Davis, S. et al. (1996) Cell 87:1161.
    4. Kim, K.-T. et al. (2005) J. Biol. Chem. 280:20126.
    5. Kugathasan, L. et al. (2009) J. Exp. Med. 206:2221.
    6. Suri, C. et al. (1998) Science 282:468.
    7. Jeansson, M. et al. (2011) J. Clin. Invest. 121:2278.
    8. Saharinen, P. et al. (2008) Nat. Cell. Biol. 10:527.
    9. Fukuhara, S. et al. (2008) Nat. Cell Biol. 10:513.
    10. Jho, D. et al. (2005) Circ. Res. 96:1282.
    11. Korff, T. et al. (2012) Cardiovasc. Res. 94:510.
    12. Kim, I. et al. (2001) Circ. Res. 89:477.
    13. Carlson, T.R. et al. (2001) J. Biol. Chem. 276:26516.
    14. Dallabrida, S.M. et al. (2005) Cardiovasc. Res. 96:e8.
    15. Samuel, S.M. et al. (2010) Diabetes 59:51.
    16. Holopainen, T. et al. (2009) Cancer Res. 69:4656.
  • Entrez Gene IDs:
    284 (Human); 11600 (Mouse)
  • Alternate Names:
    AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
    Authors: YT Ho, G Adriani, S Beyer, PT Nhan, RD Kamm, JC Kah
    Sci Rep, 2017;7(1):707.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
    Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
    Lab. Invest., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Dimerization of Tie2 mediated by its membrane-proximal FNIII domains
    Authors: JO Moore, MA Lemmon, KM Ferguson
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells
    Authors: Wilson Chan
    PLoS ONE, 2016;11(6):e0156994.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Tie1 controls angiopoietin function in vascular remodeling and inflammation
    J Clin Invest, 2016;0(0):.
  6. Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats
    Sci Rep, 2016;6(0):33428.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  7. New Insights into the Pro-Inflammatory Activities of Ang1 on Neutrophils: Induction of MIP-1? Synthesis and Release
    PLoS ONE, 2016;11(9):e0163140.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
    Sci Adv, 2016;2(4):e1501290.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Angiopoietin-1 Regulates Brain Endothelial Permeability through PTPN-2 Mediated Tyrosine Dephosphorylation of Occludin.
    Authors: Siddiqui M, Mayanil C, Kim K, Tomita T
    PLoS ONE, 2015;10(6):e0130857.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Acute Systemic Infection with Dengue Virus Leads to Vascular Leakage and Death through Tumor Necrosis Factor-alpha and Tie2/Angiopoietin Signaling in Mice Lacking Type I and II Interferon Receptors.
    Authors: Phanthanawiboon S, Limkittikul K, Sakai Y, Takakura N, Saijo M, Kurosu T
    PLoS ONE, 2015;11(2):e0148564.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  11. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
    Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
    Nat Commun, 2015;6(0):5962.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Regulation of endothelial cell proliferation and vascular assembly through distinct mTORC2 signaling pathways.
    Authors: Wang S, Amato K, Song W, Youngblood V, Lee K, Boothby M, Brantley-Sieders D, Chen J
    Mol Cell Biol, 2015;35(7):1299-313.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Angiopoietin1 inhibits mast cell activation and protects against anaphylaxis.
    Authors: Yao J, Cui M, Li M, Liu Y, He Q, Xiao J, Bai Y
    PLoS ONE, 2014;9(2):e89148.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment.
    Authors: Brunckhorst M, Xu Y, Lu R, Yu Q
    Am J Pathol, 2014;184(8):2285-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Endothelial cells from visceral adipose tissue disrupt adipocyte functions in a three-dimensional setting: partial rescue by angiopoietin-1.
    Authors: Pellegrinelli V, Rouault C, Veyrie N, Clement K, Lacasa D
    Diabetes, 2014;63(2):535-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. The RNA binding protein hnRNP-K mediates post-transcriptional regulation of uncoupling protein-2 by angiopoietin-1.
    Authors: Tahir T, Singh H, Brindle N
    Cell Signal, 2014;26(7):1379-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Vasculotide, an Angiopoietin-1 mimetic, reduces acute skin ionizing radiation damage in a preclinical mouse model.
    Authors: Korpela, Elina, Yohan, Darren, Chin, Lee Cl, Kim, Anthony, Huang, Xiaoyong, Sade, Shachar, Van Slyke, Paul, Dumont, Daniel J, Liu, Stanley
    BMC Cancer, 2014;14(0):614.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease.
    Authors: Grafton K, Moir L, Black J, Hansbro N, Hansbro P, Burgess J, Oliver B
    PLoS ONE, 2014;9(1):e85655.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils.
    Authors: Haddad L, Sirois M
    PLoS ONE, 2014;9(2):e88980.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Ligand-independent Tie2 dimers mediate kinase activity stimulated by high dose angiopoietin-1.
    Authors: Yamakawa, Daishi, Kidoya, Hiroyasu, Sakimoto, Susumu, Jia, Weizhen, Naito, Hisamich, Takakura, Nobuyuki
    J Biol Chem, 2013;288(18):12469-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Comparative analysis of human mesenchymal stem cells from bone marrow, adipose tissue, and umbilical cord blood as sources of cell therapy.
    Authors: Jin H, Bae Y, Kim M, Kwon S, Jeon H, Choi S, Kim S, Yang Y, Oh W, Chang J
    Int J Mol Sci, 2013;14(9):17986-8001.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  22. Directed evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display.
    Authors: Brindle, Nicholas, Sale, Julian E, Arakawa, Hiroshi, Buerstedde, Jean-Mar, Nuamchit, Teonchit, Sharma, Shikha, Steele, Kathryn
    J Biol Chem, 2013;288(46):33205-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. Integration and regression of implanted engineered human vascular networks during deep wound healing.
    Authors: Hanjaya-Putra D, Shen Y, Wilson A, Fox-Talbot K, Khetan S, Burdick J, Steenbergen C, Gerecht S
    Stem Cells Transl Med, 2013;2(4):297-306.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Structural basis for angiopoietin-1-mediated signaling initiation.
    Authors: Yu, Xuehong, Seegar, Tom C M, Dalton, Annamari, Tzvetkova-Robev, Dorothea, Goldgur, Yehuda, Rajashankar, Kanagala, Nikolov, Dimitar, Barton, William
    Proc Natl Acad Sci U S A, 2013;110(18):7205-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Acute administration of recombinant Angiopoietin-1 ameliorates multiple-organ dysfunction syndrome and improves survival in murine sepsis.
    Authors: David S, Park JK, Meurs M, Zijlstra JG, Koenecke C, Schrimpf C, Shushakova N, Gueler F, Haller H, Kumpers P
    Cytokine, 2011;55(2):251-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  26. Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
    Authors: Brunckhorst MK, Wang H, Lu R, Yu Q
    Cancer Res., 2010;70(18):7283-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells.
    Authors: Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T
    J. Biol. Chem., 2009;284(42):29109-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
    Authors: Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K
    Nat. Cell Biol., 2008;10(5):527-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
    Authors: Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA
    FASEB J., 2008;22(8):3010-23.
    Species: N/A
    Sample Type: N/A
    Application: WB Ctrl
  30. Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
    Authors: Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC
    Clin. Immunol., 2007;124(2):158-64.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  31. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007;25(8):1862-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. Obligatory participation of macrophages in an angiopoietin 2-mediated cell death switch.
    Authors: Rao S, Lobov IB, Vallance JE, Tsujikawa K, Shiojima I, Akunuru S, Walsh K, Benjamin LE, Lang RA
    Development, 2007;134(24):4449-58.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  33. Angiopoietin-2 levels are elevated in exudative pleural effusions.
    Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
    Chest, 2006;129(5):1259-66.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  34. Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo.
    Authors: Horst AK, Ito WD, Dabelstein J, Schumacher U, Sander H, Turbide C, Brummer J, Meinertz T, Beauchemin N, Wagener C
    J. Clin. Invest., 2006;116(6):1596-605.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  35. Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells.
    Authors: Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, Papapetropoulos A
    J. Cell. Physiol., 2006;207(2):506-11.
    Species: Bovine
    Sample Type: Cell Culture Supernates
    Application: Bioassay
  36. A neurovascular niche for neurogenesis after stroke.
    Authors: Ohab JJ, Fleming S, Blesch A, Carmichael ST
    J. Neurosci., 2006;26(50):13007-16.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  37. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
    Authors: Satchell SC, Anderson KL, Mathieson PW
    J. Am. Soc. Nephrol., 2004;15(3):566-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  38. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003;33(10):883-90.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  39. Expansion of human SCID-repopulating cells under hypoxic conditions.
    Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
    J. Clin. Invest., 2003;112(1):126-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 39 Citations
Primary Antibodies
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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