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His Tag Antibody

MAB050 36 Citations 7 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB050-SP
MAB050-100
MAB050-500
Detection of His Tag by Western Blot.
3 Images
Product Details
Citations (36)
FAQs
Supplemental Products
Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

His Tag Antibody Summary

Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.
Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
His-tagged peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunoaffinity Purification
Immobilized anti-His antibody can be used to affinity purify proteins containing exposed His tags. The column capacity must be determined for each individual His-tagged protein.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of His Tag by Western Blot. View Larger

Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged human BAI-1. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry. View Larger

Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged proteins was stained with Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer
(Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Simple Western Detection of His Tag by Simple WesternTM. View Larger

Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of CHO Chinese hamster ovary cell line transfected with His-tagged human S1P4/EDG-6, loaded at 0.5 mg/mL. A specific band was detected for His-tagged S1P4/EDG-6 at approximately 75 kDa (as indicated) using 10 µg/mL of Mouse Anti-His Tag Monoclonal Antibody
(Catalog # MAB050). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine

Product Datasheets

Product Datasheet
COA
SDS

Citations for His Tag Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation
    Authors: L Herviou, M Jourdan, AM Martinez, G Cavalli, J Moreaux
    Leukemia, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  2. Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab
    Authors: SM Chin, CR Kimberlin, Z Roe-Zurz, P Zhang, A Xu, S Liao-Chan, D Sen, AR Nager, NS Oakdale, C Brown, F Wang, Y Yang, K Lindquist, YA Yeung, S Salek-Arda, J Chaparro-R
    Nat Commun, 2018;9(1):4679.
    Species: Recombinant Protein
    Sample Types: Recombinant Protein
    Applications: ELISA capture
  3. Reciprocal negative regulation between the tumor suppressor protein p53 and B cell CLL/lymphoma 6 (BCL6) via control of caspase-1 expression
    Authors: MK Kim, JY Song, DI Koh, JY Kim, M Hatano, BN Jeon, MY Kim, SY Cho, KS Kim, MW Hur
    J. Biol. Chem., 2018;0(0):.
    Applications: WB
  4. C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor
    Authors: S Iyer, V Subramania, KR Acharya
    PeerJ, 2018;6(0):e5815.
    Species: Insect
    Sample Types: Virus
    Applications: WB
  5. Biophysical analysis of the dynamics of calmodulin interactions with neurogranin and Ca2+/calmodulin-dependent kinase II
    Authors: C Seeger, VO Talibov, UH Danielson
    J. Mol. Recognit., 2017;0(0):.
    Applications: Bioassay
  6. Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein
    Authors: JH Urban, MA Moosmeier, T Aumüller, M Thein, T Bosma, R Rink, K Groth, M Zulley, K Siegers, K Tissot, GN Moll, J Prassler
    Nat Commun, 2017;8(1):1500.
    Applications: Bioassay
  7. Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity
    Authors: D Wensel, Y Sun, Z Li, S Zhang, C Picarillo, T McDonagh, D Fabrizio, M Cockett, M Krystal, J Davis
    Antimicrob. Agents Chemother., 2017;0(0):.
    Applications: Binding Assay
  8. Structural basis of homo- and heterotrimerization of collagen I
    Authors: U Sharma, L Carrique, S Vadon-Le G, N Mariano, RN Georges, F Delolme, P Koivunen, J Myllyharju, C Moali, N Aghajari, DJ Hulmes
    Nat Commun, 2017;8(0):14671.
    Species: Human
    Sample Types: Protein
    Applications: WB
  9. Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach
    Authors: P Martinez-M, K Tran, J Guenaga, G Lindgren, M Àdori, Y Feng, GE Phad, N Vázquez Be, S Bale, J Ingale, V Dubrovskay, S O'Dell, L Pramanik, M Spångberg, M Corcoran, K Loré, JR Mascola, RT Wyatt, GB Karlsson H
    Immunity, 2017;46(5):804-817.e7.
    Species: Primate
    Sample Types: Plasma
    Applications: ELISA Capture
  10. Hypoxia favors the generation of human plasma cells
    Authors: M Schoenhals, M Jourdan, A Bruyer, A Kassambara, B Klein, J Moreaux
    Cell Cycle, 2017;0(0):1-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  11. Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis
    Sci Rep, 2016;6(0):36862.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cross-linking
  12. A Novel Bispecific Antibody Targeting EGFR and cMet that is Effective Against EGFR Inhibitor- Resistant Lung Tumors
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Func
  13. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  14. Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting
    Authors: Alexey A Lugovskoy
    MAbs, 2016;0(0):0.
    Applications: Flow
  15. I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4
    Authors: K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  16. Thermostability of Well-Ordered HIV Spikes Correlates with the Elicitation of Autologous Tier 2 Neutralizing Antibodies
    PLoS Pathog, 2016;12(8):e1005767.
    Species: Guinea Pig
    Sample Types: Serum
    Applications: ELISA Capture
  17. VB-201, an oxidized phospholipid small molecule, inhibits CD14- and Toll-like receptor-2-dependent innate cell activation and constrains atherosclerosis.
    Authors: Mendel I, Feige E, Yacov N, Salem Y, Levi I, Propheta-Meiran O, Shoham A, Ishai E, George J, Harats D, Breitbart E
    Clin Exp Immunol, 2014;175(1):126-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  18. Memo has a novel role in S1P signaling and is [corrected] crucial for vascular development.
    Authors: Kondo, Shunya, Bottos, Alessia, Allegood, Jeremy C, Masson, Regis, Maurer, Francisc, Genoud, Christel, Kaeser, Patrick, Huwiler, Andrea, Murakami, Masato, Spiegel, Sarah, Hynes, Nancy E
    PLoS ONE, 2014;9(4):e94114.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  19. Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes.
    MAbs, 2014;6(4):1059-68.
    Species: N/A
    Sample Types: Chip
  20. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.
    Authors: Madireddi S, Eun S, Lee S, Nemcovicova I, Mehta A, Zajonc D, Nishi N, Niki T, Hirashima M, Croft M
    J Exp Med, 2014;211(7):1433-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cross-linking
  21. Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon.
    Authors: Kyathanahalli, Chandras, Marks, Jason, Nye, Kennedy, Lao, Belinda, Albrecht, Eugene D, Aberdeen, Graham W, Nathanielsz, Peter W, Jeyasuria, Panchara, Condon, Jennifer
    Endocrinology, 2013;154(12):4873-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cross-linking
  22. Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells.
    Authors: Matsui T, Higashimoto Y, Taira J, Yamagishi S
    Biochem Biophys Res Commun, 2013;441(2):405-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  23. Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG.
    Authors: Rose, Rebecca, van Berkel, Patrick, van den Bremer, Ewald T, Labrijn, Aran F, Vink, Tom, Schuurman, Janine, Heck, Albert J, Parren, Paul W H
    MAbs, 2013;5(2):219-28.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  24. Novel septin 9 repeat motifs altered in neuralgic amyotrophy bind and bundle microtubules.
    Authors: Bai X, Bowen J, Knox T, Zhou K, Pendziwiat M, Kuhlenbaumer G, Sindelar C, Spiliotis E
    J Cell Biol, 2013;203(6):895-905.
    Species: Bovine
    Sample Types: Recombinant Protein
    Applications: WB
  25. Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte.
    Authors: Stephenson-Famy A, Marks J, Suresh A
    Mol. Endocrinol., 2012;26(2):320-30.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Cross-linking
  26. Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.
    Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M
    PLoS ONE, 2012;7(5):e35639.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Cross-linking
  27. A novel role for reciprocal CD30-CD30L signaling in the cross-talk between natural killer and dendritic cells.
    Authors: Simhadri VL, Hansen HP, Simhadri VR, Reiners KS, Bessler M, Engert A, von Strandmann EP
    Biol. Chem., 2011;393(1):101-6.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Cross-linking
  28. C17 prevents inflammatory arthritis and associated joint destruction in mice.
    Authors: Chao C, Joyce-Shaikh B, Grein J
    PLoS ONE, 2011;6(7):e22256.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  29. Active 1918 pandemic flu viral neuraminidase has distinct N-glycan profile and is resistant to trypsin digestion.
    Authors: Wu ZL, Ethen C, Hickey GE, Jiang W
    Biochem. Biophys. Res. Commun., 2009;379(3):749-53.
    Species: Virus
    Sample Types: Recombinant Protein
    Applications: WB
  30. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008;283(20):13874-88.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Immunopurification
  31. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008;381(4):1025-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  32. The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
    Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A
    J. Immunol., 2007;178(6):3379-89.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Cross-linking
  33. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
    Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
    Int. J. Cancer, 2006;119(2):349-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  34. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006;449(1):164-70.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: WB
  35. Receptor-ligand binding in the cell-substrate contact zone: a quantitative analysis using CX3CR1 and CXCR1 chemokine receptors.
    Authors: Lee FH, Haskell C, Charo IF, Boettiger D
    Biochemistry, 2004;43(22):7179-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  36. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein.
    Authors: Konitsiotis A, Jovanovic B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate E, Magee A
    J Biol Chem, 0;290(6):3293-307.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

View all Antibody FAQs

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Reviews for His Tag Antibody

Average Rating: 4.9 (Based on 7 Reviews)

5 Star
85.71%
4 Star
14.29%
3 Star
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His Tag Antibody
By Anonymous on 01/11/2019
Application: Cell Depl Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse
His Tag Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

293T cells were transfected with pCDNA 3.1-Control or His-RIP1 for 48h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human His Tag Antibody (MAB050). A specific band was detected for His-RIP1 at approximately 75 kDa. This experiment was conducted under reducing conditions

Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 06/20/2018
Application: Simple Western Sample Tested: Purified protein Species: Human
Simple Western His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 07/15/2017
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Mouse
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 06/28/2016
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human

cell lysates transformed

His Tag Antibody
By Anonymous on 06/28/2016
Application: WB Sample Tested: Recombinant protein Species: Human
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 10/26/2015
Application: ELISA Sample Tested: Human recombinant test antibody Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS + Tween + BSA
Dilution: used at 2ug/mL