His Tag Antibody

  (32 citations)
(6 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (32)
FAQs
Supplemental Products
Reviews
  • Specificity
    Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.
  • Source
    Monoclonal Mouse IgG1 Clone # AD1.1.10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    His-tagged peptide
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.20 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunoaffinity Purification
    Immobilized anti-His antibody can be used to affinity purify proteins containing exposed His tags. The column capacity must be determined for each individual His-tagged protein.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged human BAI-1. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged proteins was stained with Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer
(Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of CHO Chinese hamster ovary cell line transfected with His-tagged human S1P4/EDG-6, loaded at 0.5 mg/mL. A specific band was detected for His-tagged S1P4/EDG-6 at approximately 75 kDa (as indicated) using 10 µg/mL of Mouse Anti-His Tag Monoclonal Antibody
(Catalog # MAB050). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: His Tag
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.
  • Long Name:
    Histidine Tag
  • Alternate Names:
    6 His epitope tag; 6X His; 6X-His; H; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; polyHistidine; Poly-histidine
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity
    Authors: D Wensel, Y Sun, Z Li, S Zhang, C Picarillo, T McDonagh, D Fabrizio, M Cockett, M Krystal, J Davis
    Antimicrob. Agents Chemother., 2017;0(0):.
    Application: Binding Assay
  2. Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach
    Authors: P Martinez-M, K Tran, J Guenaga, G Lindgren, M Àdori, Y Feng, GE Phad, N Vázquez Be, S Bale, J Ingale, V Dubrovskay, S O'Dell, L Pramanik, M Spångberg, M Corcoran, K Loré, JR Mascola, RT Wyatt, GB Karlsson H
    Immunity, 2017;46(5):804-817.e7.
    Species: Primate
    Sample Type: Plasma
    Application: ELISA Capture
  3. Hypoxia favors the generation of human plasma cells
    Authors: M Schoenhals, M Jourdan, A Bruyer, A Kassambara, B Klein, J Moreaux
    Cell Cycle, 2017;0(0):1-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  4. Biophysical analysis of the dynamics of calmodulin interactions with neurogranin and Ca2+/calmodulin-dependent kinase II
    Authors: C Seeger, VO Talibov, UH Danielson
    J. Mol. Recognit., 2017;0(0):.
    Application: Bioassay
  5. Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein
    Authors: JH Urban, MA Moosmeier, T Aumüller, M Thein, T Bosma, R Rink, K Groth, M Zulley, K Siegers, K Tissot, GN Moll, J Prassler
    Nat Commun, 2017;8(1):1500.
    Application: Bioassay
  6. Structural basis of homo- and heterotrimerization of collagen I
    Authors: U Sharma, L Carrique, S Vadon-Le G, N Mariano, RN Georges, F Delolme, P Koivunen, J Myllyharju, C Moali, N Aghajari, DJ Hulmes
    Nat Commun, 2017;8(0):14671.
    Species: Human
    Sample Type: Protein
    Application: WB
  7. I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4
    Authors: K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Type: Complex Sample Type
    Application: Bioassay
  8. Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting
    Authors: Alexey A Lugovskoy
    MAbs, 2016;0(0):0.
    Application: Flow
  9. Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis
    Sci Rep, 2016;6(0):36862.
    Species: Human
    Sample Type: Whole Cells
    Application: Cross-linking
  10. A Novel Bispecific Antibody Targeting EGFR and cMet that is Effective Against EGFR Inhibitor- Resistant Lung Tumors
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Functional Assay
  11. Thermostability of Well-Ordered HIV Spikes Correlates with the Elicitation of Autologous Tier 2 Neutralizing Antibodies
    PLoS Pathog, 2016;12(8):e1005767.
    Species: Guinea Pig
    Sample Type: Serum
    Application: ELISA Capture
  12. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  13. Memo has a novel role in S1P signaling and is [corrected] crucial for vascular development.
    Authors: Kondo, Shunya, Bottos, Alessia, Allegood, Jeremy C, Masson, Regis, Maurer, Francisc, Genoud, Christel, Kaeser, Patrick, Huwiler, Andrea, Murakami, Masato, Spiegel, Sarah, Hynes, Nancy E
    PLoS ONE, 2014;9(4):e94114.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  14. Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes.
    MAbs, 2014;6(4):1059-68.
    Species: N/A
    Sample Type: Chip
  15. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.
    Authors: Madireddi S, Eun S, Lee S, Nemcovicova I, Mehta A, Zajonc D, Nishi N, Niki T, Hirashima M, Croft M
    J Exp Med, 2014;211(7):1433-48.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Cross-linking
  16. VB-201, an oxidized phospholipid small molecule, inhibits CD14- and Toll-like receptor-2-dependent innate cell activation and constrains atherosclerosis.
    Authors: Mendel I, Feige E, Yacov N, Salem Y, Levi I, Propheta-Meiran O, Shoham A, Ishai E, George J, Harats D, Breitbart E
    Clin Exp Immunol, 2014;175(1):126-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG.
    Authors: Rose, Rebecca, van Berkel, Patrick, van den Bremer, Ewald T, Labrijn, Aran F, Vink, Tom, Schuurman, Janine, Heck, Albert J, Parren, Paul W H
    MAbs, 2013;5(2):219-28.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  18. Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells.
    Authors: Matsui T, Higashimoto Y, Taira J, Yamagishi S
    Biochem Biophys Res Commun, 2013;441(2):405-10.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  19. Novel septin 9 repeat motifs altered in neuralgic amyotrophy bind and bundle microtubules.
    Authors: Bai X, Bowen J, Knox T, Zhou K, Pendziwiat M, Kuhlenbaumer G, Sindelar C, Spiliotis E
    J Cell Biol, 2013;203(6):895-905.
    Species: Bovine
    Sample Type: Recombinant Protein
    Application: WB
  20. Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon.
    Authors: Kyathanahalli, Chandras, Marks, Jason, Nye, Kennedy, Lao, Belinda, Albrecht, Eugene D, Aberdeen, Graham W, Nathanielsz, Peter W, Jeyasuria, Panchara, Condon, Jennifer
    Endocrinology, 2013;154(12):4873-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Cross-linking
  21. Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte.
    Authors: Stephenson-Famy A, Marks J, Suresh A
    Mol. Endocrinol., 2012;26(2):320-30.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Cross-linking
  22. Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.
    Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M
    PLoS ONE, 2012;7(5):e35639.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Cross-linking
  23. C17 prevents inflammatory arthritis and associated joint destruction in mice.
    Authors: Chao C, Joyce-Shaikh B, Grein J
    PLoS ONE, 2011;6(7):e22256.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  24. A novel role for reciprocal CD30-CD30L signaling in the cross-talk between natural killer and dendritic cells.
    Authors: Simhadri VL, Hansen HP, Simhadri VR, Reiners KS, Bessler M, Engert A, von Strandmann EP
    Biol. Chem., 2011;393(1):101-6.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Cross-linking
  25. Active 1918 pandemic flu viral neuraminidase has distinct N-glycan profile and is resistant to trypsin digestion.
    Authors: Wu ZL, Ethen C, Hickey GE, Jiang W
    Biochem. Biophys. Res. Commun., 2009;379(3):749-53.
    Species: Virus
    Sample Type: Recombinant Protein
    Application: WB
  26. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008;283(20):13874-88.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Immunopurification
  27. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008;381(4):1025-39.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  28. The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
    Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A
    J. Immunol., 2007;178(6):3379-89.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: Cross-linking
  29. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
    Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
    Int. J. Cancer, 2006;119(2):349-58.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  30. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006;449(1):164-70.
    Species: N/A
    Sample Type: Recombinant Protein
    Application: WB
  31. Receptor-ligand binding in the cell-substrate contact zone: a quantitative analysis using CX3CR1 and CXCR1 chemokine receptors.
    Authors: Lee FH, Haskell C, Charo IF, Boettiger D
    Biochemistry, 2004;43(22):7179-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Binding Assay
  32. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein.
    Authors: Konitsiotis A, Jovanovic B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate E, Magee A
    J Biol Chem, 0;290(6):3293-307.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Expand to show all Citations

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 151  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Flow , AP MAB050R 2  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 33  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 12
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002 1  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 4.8 (Based on 6 reviews)

Have you used His Tag Antibody?

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Read what people are saying who have used His Tag Antibody.

We have 6 reviews tested in 2 species: Human, Mouse.
We have 6 reviews tested in 3 applications: Western Blot, Simple Western, ELISA.

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All Species

Images Ratings Applications Species Reviewed By Date Details
Western Blot His Tag Antibody MAB050 Array MAB050
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Excellent
 WB Human Anonymous 07/23/2018
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Image Details
Western Blot His Tag Antibody MAB050
Western Blot: His Tag Antibody [MAB050].

Summary

Application Western Blot
Sample Tested 293T human embryonic kidney cell line
Species Human

Other Experimental Details

Other Experimental Details 293T cells were transfected with pCDNA 3.1-Control or His-RIP1 for 48h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human His Tag Antibody (MAB050). A specific band was detected for His-RIP1 at approximately 75 kDa. This experiment was conducted under reducing conditions
Simple Western His Tag Antibody MAB050 Array MAB050
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Excellent
 Simple Western Human Anonymous 06/20/2018
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Image Details
Simple Western His Tag Antibody MAB050
Simple Western: His Tag Antibody [MAB050].

Summary

Application Simple Western
Sample Tested Purified protein
Species Human
Western Blot His Tag Antibody MAB050 Array MAB050
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Excellent
 WB Mouse Anonymous 07/15/2017
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Image Details
Western Blot His Tag Antibody MAB050
Western Blot: His Tag Antibody [MAB050].

Summary

Application Western Blot
Sample Tested 293T human embryonic kidney cell line
Species Mouse
Very Good
 WB Human Anonymous 06/28/2016
Show

Summary

Application Western Blot
Sample Tested HEK293T human embryonic kidney cell line
Species Human

Other Experimental Details

Other Experimental Details cell lysates transformed
Western Blot His Tag Antibody MAB050 Array MAB050
Enlarge
Excellent
 WB Human Anonymous 06/28/2016
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Image Details
Western Blot His Tag Antibody MAB050
Western Blot: His Tag Antibody [MAB050].

Summary

Application Western Blot
Sample Tested Recombinant protein
Species Human
Excellent
 ELISA Human Anonymous 10/26/2015
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Summary

Application ELISA
Sample Tested Human recombinant test antibody
Species Human

Other Experimental Details

Other Experimental Details Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS + Tween + BSA
Dilution: used at 2ug/mL

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