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His Tag Antibody

Catalog #: MAB050
57 Citations 13 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB050-SP
MAB050-100
MAB050-500

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Detection of His Tag by Western Blot.
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Citations (57)
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Reviews (13)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

His Tag Antibody Summary

Specificity
Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl‑terminus.
Source
Monoclonal Mouse IgG1 Clone # AD1.1.10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
His-tagged peptide
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunoaffinity Purification
Immobilized anti-His antibody can be used to affinity purify proteins containing exposed His tags. The column capacity must be determined for each individual His-tagged protein.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of His Tag antibody by Western Blot. View Larger

Detection of His Tag by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either non-transfected or transfected with His-tagged human CIQ4 and human MYCOC and CHO Chinese hamster ovary cell line transfected with His-tagged human BAI-1. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for His Tag at approximately 32, 65, and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of His Tag antibody in HEK293 Human Cell Line Transfected with His-tagged Proteins antibody by Flow Cytometry. View Larger

Detection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged proteins was stained with Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

Flow Cytometry View Larger

SARS-Cov-2 Spike 1 His tag protein binding to ACE-2-transfected Human Cell Line is detected by His tag Antibody. Recombinant SARS-Cov-2 Spike 1 His-tagged protein (10522-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and was detected with Mouse Anti-His Allophycocyanin-conjugated Monoclonal Antibody (IC050A, filled histogram). No staining was observed in the absence of protein (open histogram). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Simple Western Detection of His Tag antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of His Tag by Simple WesternTM. Simple Western lane view shows lysates of CHO Chinese hamster ovary cell line transfected with His-tagged human S1P4/EDG-6, loaded at 0.5 mg/mL. A specific band was detected for His-tagged S1P4/EDG-6 at approximately 75 kDa (as indicated) using 10 µg/mL of Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name
Histidine Tag
Alternate Names
6 His epitope tag; 6-His Tag; 6X His Tag ; 6X His; 6x-His Tag; 6X-His; H; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His Tag; HIS; His6-Tag; polyhistidine tag; polyHistidine; Poly-histidine

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Citations for His Tag Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

57 Citations: Showing 1 - 10
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  1. Improved yield of recombinant human IFN-alpha2b from mammalian cells using heterologous signal peptide approach
    Authors: C Wilkinson, J Kyle, M Irimpen, S Stuart, S Mohandass, A Sheperd, KJ Smith, MJ Mullin
    Protein Expression and Purification, 2022-06-02;198(0):106125.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Crystal structures of pertussis toxin with NAD+ and analogs provide structural insights into the mechanism of its cytosolic ADP-ribosylation activity
    Authors: M Sakari, MT Tran, J Rossjohn, AT Pulliainen, T Beddoe, DR Littler
    The Journal of Biological Chemistry, 2022-04-01;298(5):101892.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  3. Structural dynamics of receptor recognition and pH-induced dissociation of full-length Clostridioides difficile Toxin B
    Authors: M Jiang, J Shin, R Simeon, JY Chang, R Meng, Y Wang, O Shinde, P Li, Z Chen, J Zhang
    PloS Biology, 2022-03-24;20(3):e3001589.
    Species: N/A
    Sample Types: Complex Sample Type
    Applications: ELISA (detection)
  4. ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation
    Authors: F Okumura, N Oki, Y Fujiki, R Ikuta, K Osaki, S Hamada, K Nakatsukas, N Hisamoto, T Hara, T Kamura
    Scientific Reports, 2021-10-22;11(1):20880.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. Evaluation of a genus-specific rGroEL1-524 IgM-ELISA and commercial ELISA kits during the course of leptospirosis in Thailand
    Authors: S Maneewatch, G Doungchawe, T Kalambahet, V Luvira, N Soonthornw, P Vattanatha, U Chaisri, P Adisakwatt
    Scientific Reports, 2021-10-05;11(1):19785.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  6. Optimized expression and purification of adipose triglyceride lipase improved hydrolysis and transacylation catalytic activities in vitro
    Authors: N Kulminskay, C Radler, R Viertlmayr, C Heier, P Hofer, M Colaço-Gas, RJ Owens, R Zimmermann, R Schreiber, R Zechner, M Oberer
    The Journal of Biological Chemistry, 2021-09-17;0(0):101206.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain
    Authors: E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim
    Nature Communications, 2021-04-06;12(1):2061.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Co-IP, ICC, Immunoprecipitation, Western Blot
  8. Structure and noncanonical Cdk8 activation mechanism within an Argonaute-containing Mediator kinase module
    Authors: YC Li, TC Chao, HJ Kim, T Cholko, SF Chen, G Li, L Snyder, K Nakanishi, CE Chang, K Murakami, BA Garcia, TG Boyer, KL Tsai
    Science Advances, 2021-01-15;7(3):.
    Species: Yeast
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Forward genetics identifies a novel sleep mutant with sleep state inertia and REM sleep deficits
    Authors: GT Banks, MCC Guillaumin, I Heise, P Lau, M Yin, N Bourbia, C Aguilar, MR Bowl, C Esapa, LA Brown, S Hasan, E Tagliatti, E Nicholson, RS Bains, S Wells, VV Vyazovskiy, K Volynski, SN Peirson, PM Nolan
    Sci Adv, 2020-08-12;6(33):eabb3567.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  10. New insights into the genetic basis of premature ovarian insufficiency: Novel causative variants and candidate genes revealed by genomic sequencing
    Authors: S Jaillard, K Bell, L Akloul, K Walton, K McElreavy, WA Stocker, M Beaumont, C Harrisson, T Jääskeläin, JJ Palvimo, G Robevska, E Launay, AP Satié, N Listyasari, C Bendavid, R Sreenivasa, S Duros, J van den Be, C Henry, M Domin-Bern, L Cornevin, N Dejucq-Rai, MA Belaud-Rot, S Odent, KL Ayers, C Ravel, EJ Tucker, AH Sinclair
    Maturitas, 2020-06-20;141(0):9-19.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive Immunity
    Authors: S Ols, L Yang, EA Thompson, P Pushparaj, K Tran, F Liang, A Lin, B Eriksson, GB Karlsson H, RT Wyatt, K Loré
    Cell Rep, 2020-03-24;30(12):3964-3971.e7.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  12. Enolase and dipeptidyl peptidase IV protein sub-unit vaccines are not protective against a lethal Streptococcus suis serotype 2 challenge in a mouse model of infection
    Authors: A Dumesnil, L Martelet, D Grenier, JP Auger, J Harel, E Nadeau, M Gottschalk
    BMC Vet. Res., 2019-12-10;15(1):448.
    Species: Bacteria - Streptococcus suis
    Sample Types: Recombinant Protein
    Applications: Western Blot
  13. The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation
    Authors: MER Maitland, G Onea, CA Chiasson, X Wang, J Ma, SE Moor, KR Barber, GA Lajoie, GS Shaw, C Schild-Pou
    Sci Rep, 2019-07-08;9(1):9864.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  14. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019-06-11;27(11):3117-3123.e5.
    Species: Human
    Sample Types: Cell Culture Supernates, Protein
    Applications: ELISA Development, Epitope Mapping
  15. The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors
    Authors: M Yeste-Vela, T Guo, X Mao, E Stankiewic, G Scandura, H Li, CS Wang, S Kudahetti, T Oliver, D Berney, J Shamash, YJ Lu
    Am J Cancer Res, 2019-05-01;9(5):855-871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Knockdown
  16. The HIV-1 Envelope Glycoprotein C3/V4 Region Defines a Prevalent Neutralization Epitope following Immunization
    Authors: L Lei, YR Yang, K Tran, Y Wang, CI Chiang, G Ozorowski, Y Xiao, AB Ward, RT Wyatt, Y Li
    Cell Rep, 2019-04-09;27(2):586-598.e6.
    Species: Guinea Pig
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  17. Thrombin cleaves and activates the protease-activated receptor 2 dependent on thrombomodulin co-receptor availability
    Authors: DM Heuberger, AG Franchini, J Madon, RA Schuepbach
    Thromb. Res., 2019-02-28;177(0):91-101.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development (Capture)
  18. EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation
    Authors: L Herviou, M Jourdan, AM Martinez, G Cavalli, J Moreaux
    Leukemia, 2019-02-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  19. Structure of the 4-1BB/4-1BBL complex and distinct binding and functional properties of utomilumab and urelumab
    Authors: SM Chin, CR Kimberlin, Z Roe-Zurz, P Zhang, A Xu, S Liao-Chan, D Sen, AR Nager, NS Oakdale, C Brown, F Wang, Y Yang, K Lindquist, YA Yeung, S Salek-Arda, J Chaparro-R
    Nat Commun, 2018-11-08;9(1):4679.
    Applications: ELISA (Capture)
  20. Reciprocal negative regulation between the tumor suppressor protein p53 and B cell CLL/lymphoma 6 (BCL6) via control of caspase-1 expression
    Authors: MK Kim, JY Song, DI Koh, JY Kim, M Hatano, BN Jeon, MY Kim, SY Cho, KS Kim, MW Hur
    J. Biol. Chem., 2018-11-08;0(0):.
    Applications: Western Blot
  21. C9orf72, a protein associated with amyotrophic lateral sclerosis (ALS) is a guanine nucleotide exchange factor
    Authors: S Iyer, V Subramania, KR Acharya
    PeerJ, 2018-10-17;6(0):e5815.
    Species: Insect
    Sample Types: Virus
    Applications: Western Blot
  22. rA1M-035, a physicochemically improved human recombinant ?1-microglobulin, has therapeutic effects in rhabdomyolysis-induced acute kidney injury
    Authors: B Åkerström, L Rosenlöf, A Hägerwall, S Rutardotti, J Ahlstedt, ME Johansson, L Erlandsson, M Allhorn, MG Gram
    Antioxid. Redox Signal., 2018-04-11;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance (SPR
  23. Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies
    Authors: F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada
    Cancer Cell, 2018-03-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Cross-linking
  24. Phage display and selection of lanthipeptides on the carboxy-terminus of the gene-3 minor coat protein
    Authors: JH Urban, MA Moosmeier, T Aumüller, M Thein, T Bosma, R Rink, K Groth, M Zulley, K Siegers, K Tissot, GN Moll, J Prassler
    Nat Commun, 2017-11-15;8(1):1500.
    Applications: Bioassay
  25. Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity
    Authors: D Wensel, Y Sun, Z Li, S Zhang, C Picarillo, T McDonagh, D Fabrizio, M Cockett, M Krystal, J Davis
    Antimicrob. Agents Chemother., 2017-07-25;0(0):.
    Applications: Binding Assay
  26. Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach
    Authors: P Martinez-M, K Tran, J Guenaga, G Lindgren, M Àdori, Y Feng, GE Phad, N Vázquez Be, S Bale, J Ingale, V Dubrovskay, S O'Dell, L Pramanik, M Spångberg, M Corcoran, K Loré, JR Mascola, RT Wyatt, GB Karlsson H
    Immunity, 2017-05-16;46(5):804-817.e7.
    Species: Primate
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  27. Hypoxia favors the generation of human plasma cells
    Authors: M Schoenhals, M Jourdan, A Bruyer, A Kassambara, B Klein, J Moreaux
    Cell Cycle, 2017-05-02;0(0):1-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  28. Structural basis of homo- and heterotrimerization of collagen I
    Authors: U Sharma, L Carrique, S Vadon-Le G, N Mariano, RN Georges, F Delolme, P Koivunen, J Myllyharju, C Moali, N Aghajari, DJ Hulmes
    Nat Commun, 2017-03-10;8(0):14671.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  29. Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis
    Sci Rep, 2016-11-18;6(0):36862.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cross-linking
  30. Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting
    Authors: Alexey A Lugovskoy
    MAbs, 2016-11-17;0(0):0.
    Applications: Flow Cytometry
  31. Thermostability of Well-Ordered HIV Spikes Correlates with the Elicitation of Autologous Tier 2 Neutralizing Antibodies
    PLoS Pathog, 2016-08-03;12(8):e1005767.
    Species: Guinea Pig
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  32. A Novel Bispecific Antibody Targeting EGFR and cMet that is Effective Against EGFR Inhibitor- Resistant Lung Tumors
    Cancer Res, 2016-05-23;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Functional Assay
  33. HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins
    J Immunol, 2016-05-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4
    Authors: K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley
    J Biol Chem, 2016-04-01;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  35. Heparan Sulfate Proteoglycans Are Important for Islet Amyloid Formation and Islet Amyloid Polypeptide-induced Apoptosis.
    Authors: Oskarsson M, Singh K, Wang J, Vlodavsky I, Li J, Westermark G
    J Biol Chem, 2015-04-28;290(24):15121-32.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Neutralization
  36. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein.
    Authors: Konitsiotis A, Jovanovic B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate E, Magee A
    J Biol Chem, 2014-12-12;290(6):3293-307.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Generation of a high-fidelity antibody against nerve growth factor using library scanning mutagenesis and validation with structures of the initial and optimized Fab-antigen complexes.
    MAbs, 2014-07-01;6(4):1059-68.
  38. Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.
    Authors: Madireddi S, Eun S, Lee S, Nemcovicova I, Mehta A, Zajonc D, Nishi N, Niki T, Hirashima M, Croft M
    J Exp Med, 2014-06-23;211(7):1433-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cross-linking
  39. Memo has a novel role in S1P signaling and is [corrected] crucial for vascular development.
    Authors: Kondo, Shunya, Bottos, Alessia, Allegood, Jeremy C, Masson, Regis, Maurer, Francisc, Genoud, Christel, Kaeser, Patrick, Huwiler, Andrea, Murakami, Masato, Spiegel, Sarah, Hynes, Nancy E
    PLoS ONE, 2014-04-08;9(4):e94114.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. Blockade of Fas signaling in breast cancer cells suppresses tumor growth and metastasis via disruption of Fas signaling-initiated cancer-related inflammation.
    Authors: Liu Q, Tan Q, Zheng Y, Chen K, Qian C, Li N, Wang Q, Cao X
    J Biol Chem, 2014-03-13;289(16):11522-35.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  41. VB-201, an oxidized phospholipid small molecule, inhibits CD14- and Toll-like receptor-2-dependent innate cell activation and constrains atherosclerosis.
    Authors: Mendel I, Feige E, Yacov N, Salem Y, Levi I, Propheta-Meiran O, Shoham A, Ishai E, George J, Harats D, Breitbart E
    Clin Exp Immunol, 2014-01-01;175(1):126-37.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Novel septin 9 repeat motifs altered in neuralgic amyotrophy bind and bundle microtubules.
    Authors: Bai X, Bowen J, Knox T, Zhou K, Pendziwiat M, Kuhlenbaumer G, Sindelar C, Spiliotis E
    J Cell Biol, 2013-12-23;203(6):895-905.
    Species: Bovine
    Sample Types: Recombinant Protein
    Applications: Western Blot
  43. Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells.
    Authors: Matsui T, Higashimoto Y, Taira J, Yamagishi S
    Biochem Biophys Res Commun, 2013-10-23;441(2):405-10.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  44. Cross-species withdrawal of MCL1 facilitates postpartum uterine involution in both the mouse and baboon.
    Authors: Kyathanahalli, Chandras, Marks, Jason, Nye, Kennedy, Lao, Belinda, Albrecht, Eugene D, Aberdeen, Graham W, Nathanielsz, Peter W, Jeyasuria, Panchara, Condon, Jennifer
    Endocrinology, 2013-10-18;154(12):4873-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cross-linking
  45. Human Fc receptor-like 5 binds intact IgG via mechanisms distinct from those of Fc receptors.
    Authors: Franco A, Damdinsuren B, Ise T, Dement-Brown J, Li H, Nagata S, Tolnay M
    J Immunol, 2013-04-24;190(11):5739-46.
    Species: Mouse
    Sample Types: Recombinant Proteins
    Applications: Surface Plasmon Resonance (SPR
  46. Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG.
    Authors: Rose, Rebecca, van Berkel, Patrick, van den Bremer, Ewald T, Labrijn, Aran F, Vink, Tom, Schuurman, Janine, Heck, Albert J, Parren, Paul W H
    MAbs, 2013-02-13;5(2):219-28.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  47. Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.
    Authors: Ricciardi M, Malpeli G, Bifari F, Bassi G, Pacelli L, Nwabo Kamdje AH, Chilosi M, Krampera M
    PLoS ONE, 2012-05-02;7(5):e35639.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  48. A novel role for reciprocal CD30-CD30L signaling in the cross-talk between natural killer and dendritic cells.
    Authors: Simhadri VL, Hansen HP, Simhadri VR, Reiners KS, Bessler M, Engert A, von Strandmann EP
    Biol. Chem., 2012-01-01;393(1):101-6.
    Applications: Cross-linking
  49. Antiapoptotic signaling via MCL1 confers resistance to caspase-3-mediated apoptotic cell death in the pregnant human uterine myocyte.
    Authors: Stephenson-Famy A, Marks J, Suresh A
    Mol. Endocrinol., 2011-12-22;26(2):320-30.
    Applications: Cross-linking
  50. C17 prevents inflammatory arthritis and associated joint destruction in mice.
    Authors: Chao C, Joyce-Shaikh B, Grein J
    PLoS ONE, 2011-07-25;6(7):e22256.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  51. Active 1918 pandemic flu viral neuraminidase has distinct N-glycan profile and is resistant to trypsin digestion.
    Authors: Wu ZL, Ethen C, Hickey GE, Jiang W
    Biochem. Biophys. Res. Commun., 2009-01-06;379(3):749-53.
    Species: Virus
    Sample Types: Recombinant Protein
    Applications: Western Blot
  52. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008-07-02;381(4):1025-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  53. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008-03-13;283(20):13874-88.
    Applications: Affinity Purification
  54. The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
    Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A
    J. Immunol., 2007-03-15;178(6):3379-89.
    Applications: Cross-linking
  55. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies.
    Authors: Fransson J, Tornberg UC, Borrebaeck CA, Carlsson R, Frendeus B
    Int. J. Cancer, 2006-07-15;119(2):349-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  56. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006-03-03;449(1):164-70.
    Applications: Western Blot
  57. Receptor-ligand binding in the cell-substrate contact zone: a quantitative analysis using CX3CR1 and CXCR1 chemokine receptors.
    Authors: Lee FH, Haskell C, Charo IF, Boettiger D
    Biochemistry, 2004-06-08;43(22):7179-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

  2. Can the His Tag Antibody (MAB050) recognize all His-tagged proteins?

    • MAB050 has been validated specifically for the detection of His-tags localized to the amino- or carboxyl-terminus of a peptide.  It is also expected to detect any series of 6 or more histidine residues as long as this epitope is conformationally accessible. For applications utilizing linearized proteins, it is likely that MAB050 will have access to any consecutive histidine regions.  If used with protein in its native conformation, there is the potential that the protein will be folded such that the antibody is not able to bind its target.

  3. What is the difference between His Tag Antibodies, Catalog #s MAB050 and MAB050R?

    • The primary difference is how the antibodies are produced. Catalog # MAB050R is made using recombinant DNA technology while Catalog # MAB050 is produced from a hybridoma.

View all Antibody FAQs

Isotype Controls

Mouse IgG1 Isotype Control

MAB002
252 Citations
21 Reviews
1 Image
Ctrl

Mouse IgG1 Isotype Control

MAB002R
1 Citations
Ctrl

Primary Antibodies

His Tag Antibody

MAB050R
4 Citations
8 Reviews
2 Images
WB,Flow,AP

Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
3 Reviews

Secondary Antibodies

Donkey Anti-Mouse IgG Biotinylated Antibody

BAF018
2 Citations
WB

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

NL009
10 Citations
3 Reviews
Flow,IHC,ICC

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

NL007
21 Citations
Flow,IHC,ICC

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

NL008
10 Citations
Flow,IHC,ICC

Goat Anti-Mouse IgG Biotinylated Antibody

BAF007
6 Citations
4 Reviews
WB

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

F0101B
8 Citations
1 Review
1 Image
Flow

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

F0103B
5 Citations
1 Review
1 Image
Flow

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

F0102B
15 Citations
3 Reviews
1 Image
Flow

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

F0114
1 Citations
1 Image
Flow

Mouse IgG (H+L) Antibody

D-201-C-ABS2

Mouse IgG Antibody

AF007
12 Citations
WB

Mouse IgG Fc Antibody

G-202-C
4 Citations
1 Review

Mouse IgG HRP-conjugated Antibody

HAF018
13 Citations
2 Reviews
1 Image
WB

Mouse IgG Horseradish Peroxidase-conjugated Antibody

HAF007
125 Citations
12 Reviews
2 Images
WB,IHC

Mouse IgG VisUCyte HRP Polymer Antibody

VC001
2 Citations
1 Image
IHC

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

VC002
4 Citations
2 Images
IHC

Reviews for His Tag Antibody

Average Rating: 4.8 (Based on 13 Reviews)

5 Star
76.92%
4 Star
23.08%
3 Star
0%
2 Star
0%
1 Star
0%

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His Tag Antibody
By Anonymous on 08/31/2023
Application: Flow Sample Tested: Recombinant protein Species: Mouse
Flow Cytometry His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 04/06/2022
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 10/29/2021
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 08/06/2021
Application: WB Sample Tested: Recombinant protein Species: Mouse
Western Blot His Tag Antibody MAB050
By Anonymous on 05/14/2019
Application: ELISA Sample Tested: CTLL-2 mouse cytotoxic T cell line,HT-2 mouse T cell line Species: Mouse
His Tag Antibody
By Jenna Nguyen on 05/07/2019
Application: Flow Sample Tested: 7TD1 mouse hybridoma cell line Species: Mouse
His Tag Antibody
By Jenna Nguyen on 01/11/2019
Application: Cell Depl Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse
His Tag Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

293T cells were transfected with pCDNA 3.1-Control or His-RIP1 for 48h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human His Tag Antibody (MAB050). A specific band was detected for His-RIP1 at approximately 75 kDa. This experiment was conducted under reducing conditions

Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 06/20/2018
Application: Simple Western Sample Tested: Purified protein Species: Human
Simple Western His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 07/15/2017
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Mouse
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 06/28/2016
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human

cell lysates transformed

His Tag Antibody
By Rachel Jacobson on 06/28/2016
Application: WB Sample Tested: Recombinant protein Species: Human
Western Blot His Tag Antibody MAB050
His Tag Antibody
By Anonymous on 10/26/2015
Application: ELISA Sample Tested: Human recombinant test antibody Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: TBS + Tween + BSA
Dilution: used at 2ug/mL