Recombinant Human Angiopoietin-like 6 Protein, CF Summary
Ala20-Leu470, with an N-terminal c-Myc tag
8466-AN/CF (carrier free)
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human Angiopoietin‑like Protein 6/ANGPTL6 (Catalog # 8466-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (2078-T4) binds with an ED50 of 30.0-360 ng/mL.
Background: Angiopoietin-like Protein 6/ANGPTL6
Angiopoietin-like Protein 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain and a fibrinogen-like domain (1, 2). Mature human ANGPTL6 shares 75% sequence identity with both mouse and rat ANGPTL6. ANGPTL6 is highly expressed in hepatocytes and is detectable in serum (1, 2). Within the liver, secreted ANGPTL6 inhibits gluconeogenesis and promotes insulin sensitivity and energy expenditure (3, 4). Circulating ANGPTL6 promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis (5, 6). An integrin-binding RGD motif located within the fibrinogen-like domain of ANGPTL6, enables it to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells (5, 7). Elevated levels of ANGPTL6 in serum are associated with metabolic syndrome, diabetes, and pre-eclampsia (8-11). Serum ANGPTL6 levels are also elevated during obesity (4, 12). ANGPTL6 is reported to promote the survival and expansion of hematopoietic stem cells (13). ANGPTL6 is produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells (7).
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- Akhter, S. et al. (2013) Protein Cell 4:220.
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