Recombinant Human APCDD1 His-tag Protein, CF Summary
Leu27-His492, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When 300 ng/mL of Biontinylated Recombinant Mouse Wnt-3a(Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD1 (Catalog # 10141-AP) with an ED50 of 0.6-4.8 μg/mL.
2 μg/lane of Recombinant Mouse APCDD1 His-tag (Catalog # 10141-AP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.
APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.
- Mazzoni, J. et al. (2017) Neuron. 96:1055.
- Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
- Shimomura, Y. (2010) Nature 464:1043.
- Kandimalla, R. (2017) Oncogenesis 6:e308.
- Yutaka Shimomura, et al. (2010) Nature 464:1043.
- Sung-Gook CHO, (2017) Oncology Letters 14:4845.
- Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.
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