Recombinant Human APCDD1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10141-AP-100
Recombinant Human APCDD1 His-tag Protein, CF Binding Activity
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Recombinant Human APCDD1 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When 300 ng/mL of Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD His-tag (Catalog # 10141-AP) with an ED50 of 0.6-4.8 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human APCDD1 protein
Leu27-His492, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser25 & Leu27
Predicted Molecular Mass
54 kDa
SDS-PAGE
50-70 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10141-AP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When 300 ng/mL of Biontinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) is immobilized onto Streptavidin coated plate (Catalog # CP004), it binds to Recombinant Human APCDD1 (Catalog # 10141-AP) with an ED50 of 0.6-4.8 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse APCDD1 His-tag (Catalog # 10141-AP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at  50-70 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APCDD1

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.

References
  1. Mazzoni, J. et al. (2017) Neuron. 96:1055.
  2. Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
  3. Shimomura, Y. (2010) Nature 464:1043.
  4. Kandimalla, R. (2017) Oncogenesis 6:e308.
  5. Yutaka Shimomura, et al. (2010) Nature 464:1043.
  6. Sung-Gook CHO, (2017) Oncology Letters 14:4845.
  7. Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.
Long Name
APC Down Regulated 1
Entrez Gene IDs
147495 (Human); 494504 (Mouse); 689616 (Rat)
Alternate Names
Adenomatosis polyposis coli down-regulated 1 protein; adenomatosis polyposis coli down-regulated 1; APC Down Regulated 1; APCDD1; B7323; DRAPC1; HYPT1; protein APCDD1

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