Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF

R&D Systems | Catalog # 9606-AP

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Apolipoprotein A-IV/ApoA4 Protein (9606-AP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P06727
View all Apolipoprotein A-IV/ApoA4 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Apolipoprotein A-IV/ApoA4 protein
Glu21-Ser396, with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

44 kDa

SDS-PAGE

43 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9606-AP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Apolipoprotein A-IV/ApoA4

Apolipoprotein A-IV (ApoA4) is a 45 kDa glycoprotein of the lipid transport system. Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption. Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis (1). ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well (2). ApoA4 shares several structural characteristics with ApoA1 and other exchangeable apolipoproteins. The core domain of human ApoA4 contains thirteen 22-amino acid tandem repeats, and nine of which are predicted to be amphipathic alpha -helical repeats that are critical for lipid binding and self-association (3). The overall structure of a long rod‑like dimer consisting of two 4-helix bundles stacked end-to-end in opposing orientations (4). Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

References

  1. Duverger N. et al. (1991) Eur. J. Biochem. 201:373.
  2. Maeda, N. et al. (1994) J. Biol. Chem. 269:23610.
  3. Segrest, J. P. et al. (1994). Adv. Protein. Chem. 45:303.
  4. Deng, X. et al. (2012) Structure 20:767.

Alternate Names

Apolipoprotein AIV

Entrez Gene IDs

337 (Human); 11808 (Mouse); 25080 (Rat)

Gene Symbol

APOA4

UniProt

P06727

Additional Apolipoprotein A-IV/ApoA4 Products

Product Documents for Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF

For research use only

Related Research Areas

Obesity

Citations for Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF

Customer Reviews for Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF and earn rewards!

Have you used Recombinant Human Apolipoprotein A-IV/ApoA4 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes