Recombinant Human Ataxin-3 Like Protein, CF

Recombinant Human Ataxin-3 Like Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Ataxin-3 Like

Ataxin-3-Like protein, also known as Machado-Joseph Disease (MJD) Protein-1-Like, is a 355 amino acid deubiquitinating (DUB) enzyme with a predicted molecular weight of 41 kDa.  Full-length Ataxin-3-L contains an N-terminal Joesephin Domain, two Ubiquitin-Interacting-Motifs (UIM's) and a C-terminus consisting of a polyglutamine stretch and tail.  Ataxin-3-L targets include Kruppel-Like Factor 5 (KLF5).  KLF5 has been reported to promote breast cell proliferation, survival and tumorigenesis.  Ubiquitination and proteasome-mediated degradation of KLF5 is antagonized by Ataxin-3-L, suggesting that inhibition of this DUB might provide a target for therapeutic intervention in breast cancer.  Ataxin-3 and Ataxin-3-L have been reported to cleave both K48- and K63-linked poly-Ubiquitin in vitro, though enzyme activity in such assays is inefficient when using shorter chain lengths (≤ 6).  Ataxin-3-L activity can be efficiently monitored with K63-linked tetra-Ubiquitin Rhodamine (UC-355) substrate.

Related Research Areas

• ALS Related Molecules
• Deubiquitinating Enzymes (DUBs)
• Search Ubiquitin-related Enzymes