Recombinant Human BAFF/BLyS/TNFSF13B Protein, CF

A New rhBAFF is Now Available! The new protein is competitively priced and has no tag!
New Product 7537-BF/CF.
(12 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.4-2 ng/mL in the presence of goat anti‑mouse IgM µ chain.
  • Source
    Mouse myeloma cell line, NS0-derived
    MHHHHHHHHHH GGGSGGGSGGGS IEGR Human BAFF
    (Ala134-Leu285)
    Accession # Q9Y275
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    19.6 kDa
  • SDS-PAGE
    22-29 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2149-BF/CF
 
2149-BF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BAFF/BLyS/TNFSF13B
B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature human BAFF consists of a 46 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 218 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 134-285 of the ECD, human BAFF shares 72% aa sequence identity with mouse BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg133 and Ala134 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF co-stimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).
  • References:
    1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
    2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
    3. Moore, P.A. et al. (1999) Science 285:260.
    4. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
    5. Roschke, V. et al. (2002) J. Immunol. 169:4314.
    6. Scapini, P. et al. (2003) J. Exp. Med. 197:297.
    7. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220.
    8. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948.
    9. Yu, G. et al. (2000) Nat. Immunol. 1:252.
    10. Thompson, J.S. et al. (2001) Science 293:2108.
    11. Schiemann, B. et al. (2001) Science 293:2111.
    12. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822.
    13. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
    14. Huard, B. et al. (2001) J. Immunol. 167:6225.
    15. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537.
    16. Walters, S. et al. (2009) J. Immunol. 182:793.
    17. Chang, S.K. et al. (2006) Blood 108:2687.
  • Long Name:
    B cell Activating Factor
  • Entrez Gene IDs:
    10673 (Human); 24099 (Mouse); 498666 (Rat)
  • Alternate Names:
    ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. EZH2 inhibition by tazemetostat results in altered dependency on B-cell activation signaling�in DLBCL
    Authors: D Brach, D Johnston-B, A Drew, T Lingaraj, V Motwani, NM Warholic, I Feldman, C Plescia, JJ Smith, RA Copeland, H Keilhack, E Chan-Peneb, SK Knutson, SA Ribich, A Raimondi, MJ Thomenius
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. TACI expression and signaling in chronic lymphocytic leukemia.
    Authors: Mamara, Antigoni, Germenis, Anastasi, Kompoti, Maria, Palassopoulou, Maria, Mandala, Eudokia, Banti, Anastasi, Giannakoulas, Nikolaos, Speletas, Matthaio
    J Immunol Res, 2015;2015(0):478753.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Metabolic reprogramming is required for antibody production that is suppressed in anergic but exaggerated in chronically BAFF-exposed B cells.
    Authors: Caro-Maldonado, Alfredo, Wang, Ruoning, Nichols, Amanda G, Kuraoka, Masayuki, Milasta, Sandra, Sun, Lillian, Gavin, Amanda L, Abel, E Dale, Kelsoe, Garnett, Green, Douglas, Rathmell, Jeffrey
    J Immunol, 2014;192(8):3626-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Phosphoinositide protein kinase PDPK1 is a crucial cell signaling mediator in multiple myeloma.
    Authors: Chinen Y, Kuroda J, Shimura Y, Nagoshi H, Kiyota M, Yamamoto-Sugitani M, Mizutani S, Sakamoto N, Ri M, Kawata E, Kobayashi T, Matsumoto Y, Horiike S, Iida S, Taniwaki M
    Cancer Res, 2014;74(24):7418-29.
    Species: Human
    Sample Type: whole cells
    Application: Bioassay
  5. Diversion of the host humoral response: a novel virulence mechanism of Haemophilus influenzae mediated via outer membrane vesicles.
    Authors: Deknuydt F, Nordstrom T, Riesbeck K
    J Leukoc Biol, 2014;95(6):983-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. H-Y antigen-binding B cells develop in male recipients of female hematopoietic cells and associate with chronic graft vs. host disease.
    Authors: Sahaf B, Yang Y, Arai S, Herzenberg L, Herzenberg L, Miklos D
    Proc Natl Acad Sci U S A, 2013;110(8):3005-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
    Authors: Zhu J, Sun L, Lin S
    J. Exp. Clin. Cancer Res., 2012;31(0):31.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Overexpression of the autoimmunity-associated phosphatase PTPN22 promotes survival of antigen-stimulated CLL cells by selectively activating AKT.
    Blood, 2012;119(26):6278-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. BAFF is increased in renal transplant patients following treatment with alemtuzumab.
    Authors: Bloom D, Chang Z, Pauly K
    Am. J. Transplant., 2009;9(8):1835-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model.
    Authors: Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N
    Clin. Cancer Res., 2007;13(19):5903-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
    J. Immunol., 2007;178(5):2872-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Decreased expression of tumor necrosis factor family receptors involved in humoral immune responses in preterm neonates.
    Authors: Kaur K, Chowdhury S, Greenspan NS, Schreiber JR
    Blood, 2007;110(8):2948-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 12 Citations
Primary Antibodies
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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