Recombinant Human BAI2 Protein Fc Chimera, CF

Catalog # Availability Size / Price Qty
9338-BA-050
Recombinant Human BAI2 Protein Fc Chimera Bioactivity
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Recombinant Human BAI2 Protein Fc Chimera, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 (Catalog # 9115-TN) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI2 Fc Chimera that produces 50% of the optimal binding response is approximately 25-150 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human BAI2 protein
Human BAI2
(Phe21-Asp911 )
Accession # O60241-3
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Phe21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
123 kDa
SDS-PAGE
128-200 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9338-BA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human BAI2 Protein Fc Chimera Bioactivity View Larger

When Recombinant Human C1qL3 (Catalog # 9115-TN) is coated at 2 µg/mL (100 µL/well), Recombinant Human BAI2 Fc Chimera (Catalog # 9338-BA) binds with an ED50 of 25-150 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BAI2

Brain angiogenesis inhibitor 2 (BAI2), along with BAI1 and BAI3, form a subfamily of brain specific adhesion GPCRs. They are large, glycosylated molecules, approximately 180-220 kDa, and play a role in neuronal synapse formation and maintenance. Mature human BAI2 consists of a 903 amino acid (aa) N-terminal extracellular domain (ECD) containing four TSP1 domains, a hormone binding domain (HBD), a GAIN domain and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 410 aa C-terminal cytoplasmic domain (1). Within the N-terminal ECD, human BAI2 shares 96% aa sequence identity with both mouse and rat BAI2. BAI2 is expressed primarily in neurons and astrocytes of the hippocampus and cerebral cortex in the brain (2, 3). To become functional, BAI2 might require activation by cleavage at the GPS domain (4). BAI family members are known to bind the C1q-like complement (C1qL) family via thrombospondin type 1 repeats (TSRs) which are conserved between BAI members (5). BAI2 has been shown to suppress expression of VEGF leading to increased neurogenesis in the dentate gyrus of the hippocampus (6).

References
  1. Duman, J.G. et al. (2016) Neural Plast. 2016:8301737.
  2. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
  3. Kee, H.J. et al. (2002) J. Cereb. Blood Flow Metab. 22:1054.
  4. Daisuke, O. et al. (2010) J. Recept Signal Transduct Res 30:143.
  5. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  6. Jeong, B.C. et al. (2016) FEBS Lett 580:669.
Long Name
Brain-specific Angiogenesis Inhibitor 2
Entrez Gene IDs
576 (Human); 230775 (Mouse); 313058 (Rat)
Alternate Names
BAI2; brain-specific angiogenesis inhibitor 2; Brain-specific angiongenesis inhibitor-2

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