Recombinant Human beta-Glucuronidase/GUSB Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human beta-Glucuronidase/GUSB Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to hydrolyze 4-methylumbelliferyl-beta -D-glucuronide. The specific activity is >4,000 pmol/min/μg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human beta-Glucuronidase/GUSB protein
Met1-Thr651, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
73 kDa
75-85 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 100 mM Sodium Acetate, pH 3.5
  • Recombinant Human beta ‑Glucuronidase/GUSB (rhGUSB) (Catalog # 6144-GH)
  • Substrate: 4-Methylumbelliferyl beta -D-glucuronide (Sigma, Catalog # M9130), 50 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhGUSB to 4 ng/μL in Assay Buffer.
  2. Dilute Substrate to 2 mM in Assay Buffer.
  3. Load into a plate 50 μL of 4 ng/μL rhGUSB and start the reaction by adding 50 μL of 2 mM Substrate. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of 2 mM Substrate.
  4. Read plate at excitation and emission wavelengths of 365 nm and 445 nm, respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 4-Methylumbelliferone (4-MU) (Sigma, Catalog # M1381).

Per Well:
  • rhGUSB: 0.200 μg
  • Substrate: 1 mM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: beta-Glucuronidase/GUSB

Human beta -Glucuronidase (EC encoded by the GUSB gene is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans that include heparan sulfate, chondroitin sulfate and hyaluronan (1). The enzyme is only active on the glucuronic acid of the non-reducing end. The native protein has been reported as a tetrameric glycoprotein composed of identical subunits (1, 2). Mutations in the GUSB gene are linked to mucopolysaccharidosis type VII (3). Accumulation of partially degraded glycosaminoglycans, with glucuronic acid residues at the non-reducing termini, are usually found in the lysosomes of patients with the disease (3). It has also been reported that this enzyme may contribute to the depletion of chondroitin from cartilage and thereby facilitate the damage of joints in rheumatoid arthritis (4).

  1. Shipley, J.M. et al. (1993) Am. J. Hum. Genet. 52:517.
  2. Oshima, A, et al. (1987) Proc. Natl. Acad. Sci. USA 84:685.
  3. Bell, C.E. Jr. et al. (1977) J. Clin. Invest. 59:97.
  4. Ortutay, Z. et al. (2003) Arthritis Rheum. 48:2163.
Entrez Gene IDs
2990 (Human)
Alternate Names
beta-D-glucuronidase; Beta-G1; beta-glucuronidase; BG; EC; FLJ39445; glucuronidase, beta; GUSB; MPS7

Citation for Recombinant Human beta-Glucuronidase/GUSB Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Microbial ?-glucuronidases drive human periodontal disease etiology
    Authors: Lietzan, AD;Simpson, JB;Walton, WG;Jariwala, PB;Xu, Y;Boynton, MH;Liu, J;Redinbo, MR;
    Science advances
    Species: Human
    Sample Types: Gingival Crevicular Fluid (GCF)
    Applications: Bioassay


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