Recombinant Human BMP-3b/GDF-10 Protein

Formulations:
Catalog # Availability Size / Price Qty
1543-BP-025
Product Details
Citations (2)
FAQs
Reviews

Recombinant Human BMP-3b/GDF-10 Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
E. coli-derived human BMP-3b/GDF-10 protein
Gln369-Arg478, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
disulfide-linked homodimer
Predicted Molecular Mass
12.5 kDa (monomer)

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1543-BP

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1543-BP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-3b/GDF-10

BMP-3b, also known as GDF-10, belongs to the transforming growth factor beta (TGF-beta ) superfamily which includes the TGF-beta s, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), Activins, Inhibins, Leftys, Nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs) (1). TGF-beta family members are synthesized and secreted as homodimeric or heterodimeric prepropeptides that are cleaved by proprotein convertases such as furin to generate the carboxy-terminal mature dimeric protein that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Within pro-BMP-3b, three dibasic cleavage sites have been identified. Mature BMP-3B is a disulfide-linked homodimer of the C-terminal 110 amino acids. Among TGF-beta  family members, BMP-3b is most closely related to BMP-3, sharing 83% and 30% amino acid sequence identity in their mature and pro regions, respectively (2, 3). BMP-3b is highly conserved across animal species, the aa sequence of mature human BMP-3b is 98% identical with that of the mouse or rat proteins (2, 3). BMP-3b is expressed in developing skeletal structures of the craniofacial region and the vertebral column, as well as in the adult trachea, aorta, and most abundantly in the cerebellum and uterus (2 - 4). Interestingly, in the knock-out mice, no obvious abnormalities have been found in these tissues (4). The biological function of BMP-3b is yet unknown, however studies have implicated it in the differentiation of osteoblasts, augmenting BMP-2 activity (5, 6).

References
  1. Miyazono, K. et al. (2001) J. Cell Physiol. 187:265.
  2. Cunningham, N.S. et al. (1995) Growth Factors 12:99.
  3. Takao, M. et al. (1996) Biochem. Biophys. Res. Comm. 219:656.
  4. Zhao, R. et al. (1999) Dev. Biol. 212:68.
  5. Hino, J. et al. (1999) Biochem.Biophys. Res. Comm. 256:419.
  6. Kaihara, S. et al. (2003) Life Sci. 72:1683.
Long Name
Bone Morphogenetic Protein 3b
Entrez Gene IDs
2662 (Human)
Alternate Names
BIP; BMP3b; BMP-3b; bone morphogenetic protein 3B; Bone-inducing protein; GDF10; GDF-10; growth differentiation factor 10; Growth/differentiation factor 10

Citations for Recombinant Human BMP-3b/GDF-10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. GDF10 is a signal for axonal sprouting and functional recovery after stroke.
    Authors: Li S, Nie E, Yin Y, Benowitz L, Tung S, Vinters H, Bahjat F, Stenzel-Poore M, Kawaguchi R, Coppola G, Carmichael S
    Nat Neurosci, 2015;18(12):1737-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neural Differentiation
  2. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

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