Recombinant Human BMP-4/BMP-7 Heterodimer Protein

Carrier Free

Catalog # Availability Size / Price Qty
3727-BP-010/CF

With Carrier

Catalog # Availability Size / Price Qty
3727-BP-010
Product Details
Citations (13)
FAQs
Reviews

Recombinant Human BMP-4/BMP-7 Heterodimer Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 15-75 ng/mL.
Source
E. coli-derived human BMP-4/BMP-7 Heterodimer protein
Met Human BMP-4
(Ser293-Arg408)
Accession # P12644
Human BMP-7
(Ser293-His431)
Accession # P18075
N-terminus C-terminus
N-terminal Sequence
Analysis
Met (BMP-4) & Ser293 (BMP-7)
Structure / Form
Disulfide-linked heterodimer
Predicted Molecular Mass
13.3 kDa (BMP-4), 15.8 kDa (BMP-7)

Product Datasheets

3727-BP (with carrier)

3727-BP/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3727-BP

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3727-BP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-4/BMP-7 Heterodimer

Human Bone Morphogenetic Protein 4 and 7 (BMP-4 and BMP-7), also known as osteogenic protein 1 (OP-1), belong to distinct BMP subgroups of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-4 and BMP-7 are widely expressed from early embryogenesis to adult and influence a variety of morphogenic processes (1-3). The human BMP-4 cDNA encodes a 408 amino acid (aa) precursor that includes a 19 aa signal sequence, a 273 aa propeptide, and a 116 aa mature protein (4). The human BMP-7 cDNA encodes a 431 aa precursor that includes a 29 aa signal sequence, a 263 aa propeptide, and a 139 aa mature protein (5). BMP propeptides are removed intracellulaly by proteolysis, enabling mature BMPs to form active disulfide linked homodimers or heterodimers (1). Cell types that express both BMP components can produce the heterodimers. BMP-4 and BMP-7 are each 98% conserved between human and mouse. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% aa sequence identity with other BMPs. Human BMP-7 shares approximately 60 - 70% aa sequence identity with BMP-5, -6, and -8 and less than 50% aa sequence identity with other BMPs. BMP‑4 and BMP‑7, when associated into 36 kDa heterodimers (6, 7), acquire increased potency in in vitro osteogenic differentiation assays and in vivo bone formation models compared to either homodimer (6-10). Furthermore, BMP-4/BMP-7 heterodimers induce ventral mesoderm and blood formation in mid-blastula Xenopus animal cap assays, whereas the homodimers do not (11, 12). BMP-4/BMP-7 as well as either homodimer can modulate mesoderm induction by activin and prevent subsequent dorsalization of the mesoderm (12).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Oida, S. et al. (1995) DNA Seq. 5:273.
  5. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
  6. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  7. Hazama, M. et al. (1995) Biochem. Biophys. Res. Commun. 209:859.
  8. Kusumoto, K. et al. (1997) Biochem. Biophys. Res. Commun. 239:575.
  9. Zhao, M. et al. (2005) J. Cell Biochem. 95:1.
  10. Israel, D.I. et al. (1996) Growth Factors 13:291.
  11. Suzuki, A. et al. (1997) Biochem. Biophys. Res. Commun. 232:153.
  12. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
Long Name
Bone Morphogenetic Protein 4/7
Entrez Gene IDs
652 (Human)
Alternate Names
BMP-4/BMP-7 Heterodimer

Citations for Recombinant Human BMP-4/BMP-7 Heterodimer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration
    Authors: A Krouwels, JD Iljas, AHM Kragten, WJA Dhert, FC Öner, MA Tryfonidou, LB Creemers
    Int J Mol Sci, 2020;21(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. Pbrm1 Steers Mesenchymal Stromal Cell Osteolineage Differentiation by Integrating PBAF-Dependent Chromatin Remodeling and BMP/TGF-&beta Signaling
    Authors: S Sinha, M Biswas, SS Chatterjee, S Kumar, A Sengupta
    Cell Rep, 2020;31(4):107570.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. In vitro testicular organogenesis from human fetal gonads produces fertilization-competent spermatids
    Authors: Y Yuan, L Li, Q Cheng, F Diao, Q Zeng, X Yang, Y Wu, H Zhang, M Huang, J Chen, Q Zhou, Y Zhu, R Hua, J Tian, X Wang, Z Zhou, J Hao, J Yu, D Hua, J Liu, X Guo, Q Zhou, J Sha
    Cell Res., 2020;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Feather arrays are patterned by interacting signalling and cell density waves
    Authors: WKW Ho, L Freem, D Zhao, KJ Painter, TE Woolley, EA Gaffney, MJ McGrew, A Tzika, MC Milinkovit, P Schneider, A Drusko, F Matthäus, JD Glover, KL Wells, JA Johansson, MG Davey, HM Sang, M Clinton, DJ Headon
    PLoS Biol., 2019;17(2):e3000132.
    Species: Chicken
    Sample Types: Tissue Explants
    Applications: Bioassay
  5. Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration
    Authors: I Wagner, H Wang, PM Weissert, WL Straube, A Shevchenko, M Gentzel, G Brito, A Tazaki, C Oliveira, T Sugiura, A Shevchenko, A Simon, DN Drechsel, EM Tanaka
    Dev. Cell, 2017;40(6):608-617.e6.
    Applications: Western Blot
  6. A strategy to ensure safety of stem cell-derived retinal pigment epithelium cells
    Stem Cell Res Ther, 2016;7(1):127.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Differential expression of TGF-? superfamily members and role of Smad1/5/9-signalling in chondral versus endochondral chondrocyte differentiation
    Sci Rep, 2016;6(0):36655.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. A FOXM1 Dependent Mesenchymal-Epithelial Transition in Retinal Pigment Epithelium Cells.
    Authors: Choudhary P, Dodsworth B, Sidders B, Gutteridge A, Michaelides C, Duckworth J, Whiting P, Benn C
    PLoS ONE, 2015;10(6):e0130379.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Wnt signaling interacts with bmp and edn1 to regulate dorsal-ventral patterning and growth of the craniofacial skeleton.
    Authors: Alexander C, Piloto S, Le Pabic P, Schilling T
    PLoS Genet, 2014;10(7):e1004479.
    Species: Zebrafish
    Sample Types: Embryo
    Applications: In vivo
  11. Bone morphogenetic protein signaling suppresses wound-induced skin repair by inhibiting keratinocyte proliferation and migration.
    Authors: Lewis, Christop, Mardaryev, Andrei N, Poterlowicz, Krzyszto, Sharova, Tatyana, Aziz, Ahmar, Sharpe, David T, Botchkareva, Natalia, Sharov, Andrey A
    J Invest Dermatol, 2014;134(3):827-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. Interplay between electrical activity and bone morphogenetic protein signaling regulates spinal neuron differentiation.
    Authors: Swapna, Immani, Borodinsky, Laura N
    Proc Natl Acad Sci U S A, 2012;109(40):16336-41.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: Bioassay
  13. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

FAQs

  1. What is the half-life of a recombinant or natural protein in culture at 37 °C?

    • R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

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