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Recombinant Human BMP-6, Biotinylated Protein, CF

Catalog #: BT507B/CF Datasheet / COA / SDS

Discontinued Product

BT507B/CF has been discontinued.
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Recombinant Human BMP-6, Biotinylated Protein Bioactivity
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Recombinant Human BMP-6, Biotinylated Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. In a Streptavidin coated plate (Catalog # CP004), when Biotinylated Recombinant Human BMP‑6 is used at 0.25 μg/mL, it binds Recombinant Human Activin RIA/ALK‑2 Fc Chimera (Catalog # 637-AR) with an ED50 of 0.2-1.2 μg/mL Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.02‑0.15 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human BMP-6 protein
Gln382-His513
Accession #
P22004
N-terminal Sequence
Analysis
No results obtained. Gln382 expected
Structure / Form
Disulfide-linked homodimer, biotinylated via amines
Predicted Molecular Mass
15 kDa (unlabeled)
SDS-PAGE
18, 23 and 36 kDa, reducing conditions

Product Datasheets

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BT507B/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT507B/CF

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human BMP-6, Biotinylated Protein Bioactivity View Larger

When Biotinylated Recombinant Human BMP-6 (Catalog # BT507B/CF) is used at 0.25 µg/mL, it binds Recombinant Human Activin RIA/ALK-2 Fc Chimera (Catalog # 637-AR) with an ED50 of 0.2-1.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-6

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, is a member of the BMP subfamily of TGF-beta superfamily proteins. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis (1). Human BMP-6 is synthesized as a 513 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C-terminal mature protein. Biologically active BMP-6 consists of a disulfide-linked homodimer of the mature protein, although it can also form heterodimers with mature BMP-2 (2, 3). Mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. Cellular responses to BMP-6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (4, 5). BMP-6 induces the expression of Noggin and is subsequently antagonized by Noggin (6). BMP-6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (7), chondrocyte maturation (8), Ang II-induced aldosterone production in the adrenal cortex (4), hormone production and responsiveness in ovarian granulosa cells (9), iNOS and TNF-alpha production in macrophages (5), the cell death of B cells (10), and neurite outgrowth (11). BMP-6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). It enhances tumor progression by promoting local angiogenesis and differentiation of immune tolerizing M2 macrophages (13-15). Through interactions with the BMP coreceptor RGM-C/Hemojuvelin, BMP-6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (16). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (3).

References
  1. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  3. Israel, D.I. et al. (1996) Growth Factors 13:291.
  4. Inagaki, K. et al. (2006) Endocrinology 147:2681.
  5. Hong, J.H. et al. (2008) Immunology 128:e442.
  6. Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
  7. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  8. Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
  9. Shi, J. et al. (2009) Fertil. Steril. 92:1794.
  10. Kersten, C. et al. (2005) BMC Immunol. 6:9.
  11. Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
  12. Zhang, Z. et al. (2006) Neuroscience 138:47.
  13. Dai, J. et al. (2005) Cancer Res. 65:8274.
  14. Kwon, S.J. et al. (2014) Prostate 74:121.
  15. Lee, J.-H. et al. (2013) Cancer Res. 73:3604.
  16. Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.
Long Name
Bone Morphogenetic Protein 6
Entrez Gene IDs
654 (Human); 12161 (Mouse); 25644 (Rat)
Alternate Names
BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1

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