Recombinant Human BMP-8b Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in HCl.|
|Reconstitution||Reconstitute at 250 μg/mL in 4 mM HCl.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7/OP-1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (2-4). BMP-8a and BMP-8b, produced from separate genes, share 98% aa sequence identity in human but only 74% in mouse within the mature regions. Human BMP-8b is synthesized as a 402 aa precursor protein that is cleaved between Arg263 and Ala264 to release the C-terminal mature protein. Mature human BMP-8b shares 91% and 71% aa sequence identity with mouse mature BMP-8a and -8b, respectively. BMP-8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5-7). In the mouse it cooperates with BMP-7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP-8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP-8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10-12). BMP-8b is also highly expressed in osteosarcomas and pancreatic cancer, and it contributes to tumor progression (13, 14). BMP-8b is also expressed in adipose tissue and the hypothalamus where it contributes to the regulation of energy balance and thermogenesis (15, 16).
- Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
- Chen, D. et al. (2004) Growth Factors 22:233.
- Bragdon, B. et al. (2010) Cell Signal. Oct 16 epub.
- Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
- Zhao, G.-Q. and B.L. Hogan (1996) Mech. Dev. 57:159.
- Ying, Y. and G.-Q. Zhao (2000) Biol. Reprod. 63:1781.
- Zhao, G.-Q. et al. (1998) Development 125:1103.
- Zhao, G.-Q. et al. (2001) Dev. Biol. 240:212.
- Zhao, G.-Q. et al. (1998) Genes Dev. 10:1657.
- van der Horst, G. et al. (2002) Bone 31:661.
- Cho, T.-J. et al. (2002) J. Bone Miner. Res. 17:513.
- Paic, F. et al. (2009) Bone 45:682.
- Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.
- Cheng, Z. et al. (2014) Oncol. Rep. 32:1861.
- Whittle, A.J. et al. (2012) Cell 149:871.
- Martins, L. et al. (2016) Cell Rep. 16:2231.
Citations for Recombinant Human BMP-8b Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Regulation of both transcription and RNA turnover contribute to germline specification
Authors: K Tan, MF Wilkinson
Sample Types: Whole Cells
Obesity induces resistance to central action of BMP8B through a mechanism involving the BBSome
Authors: E Rial-Pensa, O Freire-Agu, M Ríos, DF Guo, C Contreras, P Seoane-Col, S Tovar, R Nogueiras, C Diéguez, K Rahmouni, M López
Molecular Metabolism, 2022;0(0):101465.
Sample Types: In Vivo
Applications: In Vivo
Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells
Authors: R Zhao, Q Zuo, X Yuan, K Jin, J Jin, Y Ding, C Zhang, T Li, J Jiang, J Li, M Zhang, X Shi, H Sun, Y Zhang, Q Xu, G Chang, Z Zhao, B Li, X Wu, Y Zhang, J Song, G Chen, B Li
Nature Communications, 2021;12(1):2989.
Sample Types: Whole Cells
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