Recombinant Human C-Reactive Protein/CRP Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
1707-CR-200/CF
Product Details
Citations (6)
FAQs
Reviews

Recombinant Human C-Reactive Protein/CRP Protein, CF Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human (rh) Fc gamma RIIA at 2 µg/mL (100 µL/well) can bind rhCRP with a linear range of 0.15-10 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human C-Reactive Protein/CRP protein
Phe17-Pro224
Accession #
N-terminal Sequence
Analysis
Phe17
Structure / Form
Homopentamer
Predicted Molecular Mass
23 kDa (monomer)
SDS-PAGE
26 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1707-CR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and CaCl2.
Reconstitution Reconstitute at 200 μg/mL in sterile 20 mM Tris-HCl, pH 8.0.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1707-CR

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl, NaCl and CaCl2 with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile 20 mM Tris-HCl, pH 8.0 containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C-Reactive Protein/CRP

CRP is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are non-covalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively.

CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL-6, IL-1 and glucocorticoids are the major inducer of the CRP gene. In response to infection, inflammation or tissue damage, the level of CRP in human serum can increase 1,000-fold within 24-48 hours. It will come back to base level of less than 1 μg/mL very fast. Human CRP is an acute-phase serum protein that plays a role in the first line in host innate host defense. Like other pentraxins, CRP exhibits Ca++‑dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phogocytotic responses. In addition to phogocytosis, CRP also can induce production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF‑ alpha  by monocytes. With these functions, human CRP is an important serum protein for anti-bacterial pathogen and clearance of damaged and apoptotic cells. However, in mouse, CRP is expressed at very low level and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is the major acute phase serum protein in mice. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

References
  1. Gotschlich, E.C. and G.M. Edelman (1965) Proc. Natl. Acad. Sci. USA 54:558.
  2. Volanakis, J.E. (2001) Molecular Immunology 38:189.
  3. Bharadwaj, D. et al. (1999) J. Experimental Medicine 190:585.
  4. Ballou S.P. and G. Lozanski (1992) Cytokine 4:361.
  5. Danesh, J. et al. (2004) N. Engl. J. Med. 350:1387.
Entrez Gene IDs
1401 (Human); 12944 (Mouse); 25419 (Rat)
Alternate Names
C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895

Citations for Recombinant Human C-Reactive Protein/CRP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Impact of C-reactive protein on osteo-/chondrogenic transdifferentiation and calcification of vascular smooth muscle cells
    Authors: LA Henze, TTD Luong, B Boehme, J Masyout, MP Schneider, S Brachs, F Lang, B Pieske, A Pasch, KU Eckardt, J Voelkl, I Alesutan
    Aging (Albany NY), 2019;11(15):5445-5462.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. C-Reactive Protein Promotes Diabetic Kidney Disease in db/db Mice via the CD32b-Smad3-mTOR signaling Pathway
    Sci Rep, 2016;6(0):26740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. CRP enhances soluble LOX-1 release from macrophages by activating TNF-alpha converting enzyme.
    Authors: Zhao XQ, Zhang MW, Wang F, Zhao YX, Li JJ, Wang XP, Bu PL, Yang JM, Liu XL, Zhang MX, Gao F, Zhang C, Zhang Y
    J. Lipid Res., 2011;52(5):923-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011;54(10):2713-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
    Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
    J. Leukoc. Biol., 2008;84(4):1109-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. C-reactive protein impairs human CD14+ monocyte-derived dendritic cell differentiation, maturation and function.
    Authors: Zhang R, Becnel L, Li M, Chen C, Yao Q
    Eur. J. Immunol., 2006;36(11):2993-3006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. Is Recombinant Human C-Reactive Protein, Catalog # 1707-CR, a monomer or homopentamer?

    • This protein runs at approximately 26 kDa (monomer) under both reducing and non-reducing conditions on SDS-PAGE. However, from HPLC results, this protein will form a homopentamer, with a sole peak at 102-105kDa. 

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