hh Recombinant Human C1qTNF14/C1qL1, CF 9134-TN-050: R&D Systems

Recombinant Human C1qTNF14/C1qL1, CF

  
  • Purity
    >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human C1qTNF14/C1qL1 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI3 (Catalog # 9106-BA) that produces 50% of the optimal binding response is approximately 0.4-2.4 μg/mL
  • Source
    E. coli-derived Thr125-Asp258, with an N-terminal Met and 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Structure / Form
    Noncovalently-linked homotrimer
  • Predicted Molecular Mass
    16 kDa
  • SDS-PAGE
    13 kDa, reducing conditions
9134-TN
 
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Reconstitution Reconstitute at 1 mg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human C1qTNF14/C1qL1 (Catalog # 9134-TN) is coated at 1 µg/mL (100 μL/well), Recombinant Human BAI3 (Catalog #
(Catalog # 9106-BA) binds with a typical ED50 of 0.4-2.4 μg/mL.
Background: C1qL1
C1qTNF14 (CTRP14), also known as C1qL1, is an approximately 30 kDa member of the C1q family of secreted proteins (1, 2). Mature human C1qTNF14 contains a collagen-like region and one C1q-like domain and can form disulfide-linked heteromers with C1qTNF11/C1qL4 (3, 4). Within the C1q-like domain, human C1qTNF14 shares 100% aa sequence identity with mouse and rat C1qTNF14. C1qTNF14 is expressed in the inferior olive, hippocampus, and cerebral cortex (5). Similarly to C1qTNF13/C1qL3, C1qTNF10/C1qL2, and C1qTNF11/C1qL4, C1qTNF14 binds to BAI3 in the cerebral cortex and on cerebellar Purkinje cells (5-8). C1qTNF14/C1qL1 binding to BAI3 induces the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (5, 7).
  • References:
    1. Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
    2. Yuzaki, M. (2010) Eur. J. Neurosci. 32:191.
    3. Ressl, S. et al. (2015) Structure 23:688.
    4. Wei, Z. et al. (2013) J. Biol. Chem. 288:10214.
    5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
    6. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
    7. Kakegawa, W. et al. (2015) Neuron 85:316.
    8. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
  • Long Name:
    Complement Component 1, Q Subcomponent-Like 1
  • Entrez Gene IDs:
    10882 (Human); 23829 (Mouse); 363686 (Rat)
  • Alternate Names:
    C1qL1; C1q-related factor; C1QRF; C1QRFCRFC1QTNF14; C1qTNF14; Complement component 1 Q subcomponent-like 1; complement component 1, q subcomponent-like 1; CTRP14
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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