Recombinant Human CACHD1 Fc Chimera Protein, CF

R&D Systems | Catalog # 10749-CA

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human CACHD1 Fc Chimera Protein (10749-CA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q5VU97.2

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CACHD1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CACHD1 protein
Human CACHD1
(Glu36-Pro1095)
Accession # Q5VU97.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu36

Predicted Molecular Mass

145 kDa

SDS-PAGE

133-147 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human CACHD1 Fc Chimera (Catalog # 10749-CA) binds Recombinant Human/Mouse Wnt-5a Biotinylated (Catalog # BT645). The ED50 for this effect is 0.08-0.640 μg/mL.

Scientific Data Images for Recombinant Human CACHD1 Fc Chimera Protein, CF

Recombinant Human CACHD1 Fc Chimera Protein Binding Activity.

Recombinant Human CACHD1 Fc Chimera (Catalog # 10749-CA) binds Recombinant Human/Mouse Wnt-5a Biotinylated Protein (BT645). The ED50 for this effect is 0.08-0.640 μg/mL.

Recombinant Human CACHD1 Fc Chimera Protein SDS PAGE.

2 μg/lane of Recombinant Human CACHD1 Fc Chimera Protein (Catalog # 10749-CA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 133-147 kDa and 260-300 kDa, respectively.

Formulation, Preparation, and Storage

10749-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CACHD1

CACHD1 (Ca2+ channel and chemotaxis receptor Domain Containing 1) is a alpha 2 delta -Like Protein that modulates CaV3 voltage‑gated calcium channel activity (1, 2). Mature Human CACHD1 consists of a 1060 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 158 aa cytoplasmic domain. The ECD contains a von Willebrand factor A (VWA) domain and two bacterial chemosensory-like cache domains (1). Within the ECD, human CACHD1 shares 97.5% and 97.7% aa sequence identity with mouse and rat CACHD1, respectively. Diseases associated with CACHD1 include Cercarial Dermatitis and Hypertropia. CACHD1 increases the presence of CaV3.1 at the cell surface and causes an increase in channel open probability (2). CACHD1 is a new activity-modifying protein for voltage‑gated calcium channels (2). Such physiological actions may have implications in targeting diseases involving aberrant neuronal firing, as CaV3 channels have been proposed as therapeutic targets to combat pain and epilepsy (2). CACHD1 and MDGA1 are novel in vivo substrates for beta-site APP cleaving enzyme 1 (BACE1), suggesting that cleavage of both proteins may contribute to the numerous functions of BACE1 in the nervous system (3). BACE1 inhibition is a major therapeutic approach for Alzheimer disease (3). CACHD1 closely interacts with voltage‑gated calcium channels of the N-type58 and T-type59 at the cell surface and enhances the densities of their calcium currents (3). BACE1 cleavage is a mechanism to control CACHD1's function in calcium signaling and synaptic transmission (3). Expression of CACHD1 increased both CaV2.2 currents and cell surface trafficking in both cell lines and neurons (4). CACHD1 competed with a2d-1 for binding to CaV2.2 and for its functional effects and can therefore inhibit responses to a2d-1 (4). CACHD1 is widely expressed in many tissues, including the brain, lungs, and small intestine (4). CACHD1 contains a VWA domain that has a disrupted metal ion-dependent adhesion site (MIDAS) motif, a sequence that is conserved in the human, rat, mouse, and zebrafish CACHD1 proteins (4). At the Wnt Signaling Gordon Conference in August 2019, Yvonne Jones (Oxford) presented an unpublished crystal structure of the transmembrane proteins CACHD1 in a complex between LRP6 and Fz5. Their data suggest that CACHD1 is an inhibitor of Wnt signaling.

References

  1. Cottrell, G.S. et al. (2018) J. Neurosci. 38:9186.
  2. Stephens, G.J. and G.S. Cottrell (2019) Channels (Austin) 13:120.
  3. Njavro, J.R. et al. (2020) The FASEB J. 34:2465.
  4. Dahimene, S. et al. (2018) Cell Reports 25:1610.

Long Name

Cache Domain Containing 1

Alternate Names

VWCD1

Entrez Gene IDs

57685 (Human); 320508 (Mouse)

Gene Symbol

CACHD1

UniProt

Q5VU97.2

Additional CACHD1 Products

Product Documents for Recombinant Human CACHD1 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CACHD1 Fc Chimera Protein, CF

For research use only

Customer Reviews for Recombinant Human CACHD1 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CACHD1 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human CACHD1 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes