Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF

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Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. When 5 x 104 cells/well are added to Recombinant Human Pro-Cadherin‑12 Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately >30% will adhere after 30 minutes at 37 °C.
Optimal concentration depends on cell type as well as the application or research objectives.
Mouse myeloma cell line, NS0-derived human Cadherin-12 protein
Human Pro-Cadherin-12
Accession # P55289
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
92.5 kDa (monomer)
115 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate, NaCl and CaCl2.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Cadherin-12

The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin-12, also known as brain‑cadherin and N-cadherin 2, is a 150 kDa classical cadherin. Classical family molecules are modular in their extracellular region, mediating calcium‑dependent cell-cell adhesion through their five EC Ca++-binding repeats (2). Cadherin-12 can be further identified as a type II classical cadherin, due to the absence of a His-Ala-Val motif in its most N-terminal cadherin repeat (3). Human Cadherin-12 is synthesized as a 794 amino acid (aa) type I transmembrane preproprotein that contains a 23 aa signal peptide, a 31 aa prosequence, a 555 aa extracellular region, a 28 aa transmembrane segment, and a 157 aa cytoplasmic domain (4, 5). The five EC cadherin domains are approximately 110 aa in length and generate two beta -sheets that are oriented like bread in a sandwich. Human Cadherin‑12 EC region is 96% aa identical to mouse Cadherin-12 EC region. Cadherin-12 is expressed specifically in CNS neurons. The bulk of its expression is postnatal, and it is proposed to be involved in synaptogenesis (4). As a classic cadherin, Cadherin-12 will form homodimers and promote intercellular adhesion with itself and, possibly, cadherins-8 and -14 (6).

  1. Koch, A.W. et al. (2004) Cell. Mol. Life Sci. 61:1884.
  2. Angst, B.D. et al. (2001) J. Cell Sci. 114:629.
  3. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
  4. Selig, S. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2398.
  5. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
  6. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
Entrez Gene IDs
1010 (Human)
Alternate Names
Br-Cadherin; cadherin 12, type 2 (N-cadherin 2); Cadherin12; Cadherin-12; CDH12; CDHB; FLJ34857; N-Cadherin 2; Neural type cadherin 2; neural type, 2; neuronal cadherin 2

Citation for Recombinant Human Cadherin-12 (CAD-12) Fc Chimera, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cadherin-11 promotes the metastasis of prostate cancer cells to bone.
    Authors: Chu K, Cheng CJ, Ye X, Lee YC, Zurita AJ, Chen DT, Yu-Lee LY, Zhang S, Yeh ET, Hu MC, Logothetis CJ, Lin SH
    Mol. Cancer Res., 2008;6(8):1259-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay


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