Carbonic anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (2). Topics in a CA meeting (6th International Conference on the CAs, June 20-25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VII encoded by the CA7 gene is a cytosolic protein predominantly expressed in the salivary gland (1). CA7 may have additional tissue distributions and functions. For example, studies with CA inhibitors provide evidence that human CA7 is the CA isozyme responsible for the anticonvulsant/antiepileptic activity of sulfonamides and sulfamates (3). In fact, CA7 has been shown in a rat model to act as a developmental switch in GABAergic signaling in neurons (4, 5). The amino acid sequence of human CA7 is 95%, 94%, 89%, 76%, 72% and 70% identical to that of bovine, rat/mouse, canine, chicken, Xenopus, and zebrafish.
Recombinant Human Carbonic Anhydrase VII Protein, CF
R&D Systems | Catalog # 3177-CA
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human Carbonic Anhydrase VII Protein (3177-CA)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human Carbonic Anhydrase VII/CA7 protein
Thr2-Ala264, with a C-terminal 10-His tag
Thr2-Ala264, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr2
Predicted Molecular Mass
31 kDa
SDS-PAGE
31 kDa, reducing conditions
Activity
Measured by its esterase activity.
The specific activity is >20 pmol/min/µg, as measured under the described conditions.
The specific activity is >20 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
3177-CA
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Carbonic Anhydrase VII/CA7
References
- Montgomery, J.C. et al. (1991) Genomics 11:835.
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Vullo, D. et al. (2005) Bioorg. Med. Chem. Lett. 15:971.
- Ruusuvuori, E. et al. (2004) J. Neurosci. 24:2699.
- Rivera, C. et al. (2005) J. Physiol. 562:27.
Alternate Names
CA7
Entrez Gene IDs
766 (Human)
Gene Symbol
CA7
UniProt
Additional Carbonic Anhydrase VII/CA7 Products
Product Documents for Recombinant Human Carbonic Anhydrase VII Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Carbonic Anhydrase VII Protein, CF
For research use only
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Protocols
View specific protocols for Recombinant Human Carbonic Anhydrase VII Protein, CF (3177-CA):
Materials
- Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5
- Recombinant Human Carbonic Anhydrase VII/CA7 (rhCA7) (Catalog # 3177-CA)
- Substrate: 4-Nitrophenyl Acetate (4-NPA) (Sigma, Catalog # N8130), 100 mM stock in acetone
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhCA7 to 40 ng/µL in Assay Buffer.
- Dilute Substrate to 2 mM in Assay Buffer.
- Load in plate, 50 µL of 50 ng/µL rhCA7, and start the reaction by adding 50 µL of 2 mM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 2 mM Substrate.
- Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).
- rhCA7: 2 µg
- Substrate: 1 mM
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