Recombinant Human Carbonic Anhydrase X/CA10 Protein, CF Summary
Ala21-Asn300, with a C-terminal 10-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Carbonic Anhydrase X/CA10
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA10, also known as CA-related protein, CA-RA X, is a secreted protein without CA activity (i.e., the reversible hydration of CO2) due to point mutations in the zinc-binding site. The human and mouse CA10 are expressed in the brain, indicating that they may play a role in brain development (2, 3).
- Hewett-Emmett, D. and R.E Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Okamoto, N. et al. (2001) Biochim. Biophys. Acta 1518:311.
- Taniuchi, K. et al. (2002) Brain Res. Mol. Brain Res. 109:207.
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