Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA13 is a cytosolic enzyme with a unique and widespread distribution pattern as compared to the other cytosolic CAs, indicating that it may play important physiological roles in several organs (2).
Recombinant Human Carbonic Anhydrase XIII Protein, CF
R&D Systems | Catalog # 2194-CA
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human Carbonic Anhydrase XIII Protein (2194-CA)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human Carbonic Anhydrase XIII/CA13 protein
Ser2-His262, with a C-terminal 10-His tag
Ser2-His262, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser2
Predicted Molecular Mass
31 kDa
SDS-PAGE
33 kDa, reducing conditions
Activity
Measured by its esterase activity.
The specific activity is >5 pmol/min/µg, as measured under the described conditions.
The specific activity is >5 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
2194-CA
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 1 mg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: Carbonic Anhydrase XIII/CA13
References
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Lehtonen, J. et al. (2003) J. Biol. Chem. 279: 2719.
Alternate Names
CA13
Entrez Gene IDs
377677 (Human)
Gene Symbol
CA13
UniProt
Additional Carbonic Anhydrase XIII/CA13 Products
Product Documents for Recombinant Human Carbonic Anhydrase XIII Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Carbonic Anhydrase XIII Protein, CF
For research use only
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Protocols
View specific protocols for Recombinant Human Carbonic Anhydrase XIII Protein, CF (2194-CA):
Materials
- Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5
- Recombinant Human Carbonic Anhydrase XIII/CA13 (rhCA13) (Catalog # 2194-CA)
- Substrate: 4-Nitrophenyl Acetate (4-NPA) (Sigma, Catalog # N8130), 100 mM stock in acetone
- 96-well Clear Plate (Costar, Catalog # 92592)
- Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rhCA13 to 100 ng/µL in Assay Buffer.
- Dilute Substrate to 2.0 mM in Assay Buffer.
- In a plate load 50 µL of 100 ng/µL rhCA13, and include a Substrate Blank containing 50 µL Assay Buffer.
- Start the reaction by adding 50 µL of 2.0 mM Substrate to wells containing rhCA13 and Assay Buffer.
- Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).
- rhCA13: 5 µg
- Substrate: 1.0 mM
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