Recombinant Human Caspr1 His-tag Protein, CF Summary
Trp20-Gly1280, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in PBS.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human Caspr1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-165 kDa.
Caspr1 (contactin-associated protein 1) is a 190 kDa neuronal transmembrane protein that belongs to the neurexin superfamily. It consists of a large extracellular domain (ECD), a single transmembrane region, and a short intracellular region. The mature ECD region contains a factor VII/discoidin region, a laminin G domain, and a fibrinogen domain along with additional laminin G and epidermal growth factor domains. The intracellular domain includes a proline-rich sequence capable of binding to a subclass of SH3 domains of specific molecular signals, including Src and Fyn tyrosine kinases (1). The mature ECD of human Caspr1 shares 94% amino acid identity with both the mouse and rat proteins. Caspr1 is located at the paranodes of the myelinated axons and plays an important role in the formation and stability of this structure. It forms a complex with contactin and NFASC-155 (NF-155), acts as a barrier between the nodes of Ranvier and internodes, and is involved in the propagation of action potentials and mediation of signal transport (2). It may mediate the timing of neuron and astrocyte differentiation in neural progenitor cells (3). Caspr1 may form a g-secretase associated complex, is distributed around amyloid plaques in the cerebral cortex of mice interacting with amyloid precursor proteins, thereby potentially be involved in the pathology of Alzheimer's disease (4, 5).
- Poliak, S. et al. (1999) Neuron 24:1037.
- Sherman, D. L. et al. (2005) Neuron 48:737.
- Wu, Z. Q. et al. (2017) Cereb. Cortex. 27:1369.
- Hur, J.Y. et al. (2012) J. Biol. Chem. 287:11991.
- Zou, Y. et al. (2017) Neural Regen. Res. 12:1551.
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