Recombinant Human CCL18/PARC Protein

R&D Systems | Catalog # 394-PA

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human CCL18/PARC Protein (394-PA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P55774.1

Applications

Bioactivity
View all CCL18/PARC Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CCL18/PARC protein
Ala21-Ala89

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

7.8 kDa

Activity

Measured by its ability to inhibit Eotaxin-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR3. Nibbs, RJB. et al. (2000) J. Immunol. 164:1488.
The ED50 for this effect is 1-5 µg/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 394-PA in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 394-PA
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Carrier Free: 394-PA/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL18/PARC

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM‑CSF/IL‑4‑induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.

References

  1. Adema, G. et al. (1997) Nature 387:713.
  2. Kodelja, V. et al. (1998) J. Immunol. 160:141.

Alternate Names

AMAC-1, DC-CK1, MIP-4, PARC

Entrez Gene IDs

6362 (Human)

Gene Symbol

CCL18

UniProt

P55774.1

Additional CCL18/PARC Products

Product Documents for Recombinant Human CCL18/PARC Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 394-PA (with carrier)
Download SDS 394-PA/CF (carrier free)

Product Specific Notices for Recombinant Human CCL18/PARC Protein

For research use only

Citations for Recombinant Human CCL18/PARC Protein

Customer Reviews for Recombinant Human CCL18/PARC Protein (2)

4.5 out of 5
2 Customer Ratings
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  • Recombinant Human CCL18/PARC Protein
    Name: Anonymous
    Application: ELISA standard
    Verified Customer | Posted 06/09/2020
    We used this protein in an in-house ELISA along with mAb (394) and pAb (AF394) to detect CCL18 in human serum and plasma. This combination detected CCL18 in human serum and plasma efficiently and generated a good standard curve.
    Recombinant Human CCL18/PARC Protein 394-PA
  • Recombinant Human CCL18/PARC Protein
    Name: Balaji Mahender
    Application: Immunoassay Standard
    Verified Customer | Posted 02/05/2018

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