MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is one of many novel beta chemokines identified through bioinformatics. MIP-3 beta cDNA encodes a 98 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is cleaved to form the 77 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity) and the gene for MIP-3 beta has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for cultured human lymphocytes. MIP-3 beta has also been shown to be a unique functional ligand for CCR-7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. EBI1 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.
Recombinant Human CCL19/MIP-3 beta Protein
R&D Systems | Catalog # 361-MI
Key Product Details
- R&D Systems E. coli-derived Recombinant Human CCL19/MIP-3 beta Protein (361-MI)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Gly22-Ser98
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 3-15 ng/mL.
Reviewed Applications
Read 2 reviews rated 5 using 361-MI in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 361-MI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 361-MI/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: CCL19/MIP-3 beta
References
- Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Alternate Names
Gene Symbol
UniProt
Additional CCL19/MIP-3 beta Products
Product Documents for Recombinant Human CCL19/MIP-3 beta Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human CCL19/MIP-3 beta Protein
For research use only
Citations for Recombinant Human CCL19/MIP-3 beta Protein
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Customer Reviews for Recombinant Human CCL19/MIP-3 beta Protein (2)
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Customer Images
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Application: Cell migration/motilitySpecies: HumanVerified Customer | Posted 04/24/2025Comparison of NK cell count/µL in the control condition (R10) vs CCL19 at 1µg/mL200.000 sorted NK cells were place in the upper chamber of a 24 well plate with transwell inserts (5µm pore size). 650µL of culture media containing 1µg/mL of CCL19 were placed in the lower chamber. Cell count was performed by flow cytometry using counting bead after one hour of migration at 37ºC.
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Verified Customer | Posted 04/24/2017
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Associated Pathways
