Recombinant Human CCL23/Ck beta 8-1 (aa 46-137) Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
508-CK-025/CF
Product Details
Citations (4)
FAQs
Reviews

Recombinant Human CCL23/Ck beta 8-1 (aa 46-137) Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.01‑0.04 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.002‑0.01 µg/mL.
Source
E. coli-derived human CCL23/Ck beta 8-1 protein
Met46-Asn137
Accession #
N-terminal Sequence
Analysis
Met46
Predicted Molecular Mass
10.5 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

508-CK/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

508-CK

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL23/Ck beta 8-1

CCL23 (SCY A23), a splice variant of CK beta 8 (also known as MPIF-1), is a beta chemokine isolated from the THP-1 cDNA library. CCL23 cDNA encodes a 137 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide. Compared to CK beta 8-1, CK beta 8/MPIF-1 lacks a 17 aa residue stretch (Leu 47 - Gly 63) present in CCL23 and has a unique arginine at residue 46. CK beta 8-1/CK beta 8 (MPIF-1) and leukotactin/MIP-1δ form a subgroup of beta chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. CCL23 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1δ), and the two genes have been localized to human chromosome 17 within 200 kb of each other. CCL23 mRNA expression has been detected in the pancreas, heart and skeletal muscle. Both CK beta 8-1 and CK beta 8 (MPIF-1) are potent agonists of CCR1 and have been shown to chemoattract peripheral blood lymphocytes and monocytes. The two chemokines have also been reported to chemoattract neutrophils and to inhibit colony formation by human hematopoietic progenitor cells. Similar to CK beta 8 (MPIF-1), the truncated form of CK beta 8-1 (aa 46 - 132) produced at R&D Systems has been shown to have greatly enhanced biological activity compared to the full length mature CK beta 8-1 (aa 22 - 132).

References
  1. Youn, B-S. et al. (1998) Blood 91:3118.
  2. Macphee, C.H. et al. (1998) J. Immunol. 161:6273.
Entrez Gene IDs
6368 (Human)
Alternate Names
C6 beta-chemokine; CCL23/Ck beta 8-1; chemokine (C-C motif) ligand 23; Ck beta 8-1; CKb8; Ckb-8; Ckb81; CK-beta-8; hmrp-2a; Macrophage inflammatory protein 3; MIP3; MIP-3CK-BETA-8; MPIF1; MPIF-1Ckb-8-1; Myeloid progenitor inhibitory factor 1; SCYA23C-C motif chemokine 23; small inducible cytokine subfamily A (Cys-Cys), member 23; Small-inducible cytokine A23

Citations for Recombinant Human CCL23/Ck beta 8-1 (aa 46-137) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. CK beta 8/CCL23 induces cell migration via the Gi/Go protein/PLC/PKC delta/NF-kappa B and is involved in inflammatory responses.
    Authors: Kim J, Kim YS, Ko J
    Life Sci., 2010;86(9):300-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Proinflammatory proteases liberate a discrete high-affinity functional FPRL1 (CCR12) ligand from CCL23.
    Authors: Miao Z, Premack BA, Wei Z, Wang Y, Gerard C, Showell H, Howard M, Schall TJ, Berahovich R
    J. Immunol., 2007;178(11):7395-404.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  4. FPRL-1 induces modifications of migration-associated proteins in human neutrophils.
    Authors: Boldt K, Rist W, Weiss SM, Weith A, Lenter MC
    Proteomics, 2006;6(17):4790-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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