Recombinant Human CCL23/MPIF-1 (aa 46-120) Protein

Formulations:
Catalog # Availability Size / Price Qty
131-M1-025
Product Details
Citations (1)
FAQs
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Recombinant Human CCL23/MPIF-1 (aa 46-120) Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.70-3.5 ng/mL. This 46‑120 aa variant of CCL23 is significantly more active than the 22‑120 aa variant (Catalog # 371-MP). The ED50 of the 22‑120 aa variant on hCCR1 transfected BaF3 cells is 20-100 ng/mL.
Source
E. coli-derived human CCL23/MPIF-1 protein
Arg46-Asn120
Accession #
N-terminal Sequence
Analysis
Arg46
Predicted Molecular Mass
8.6 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

131-M1

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

131-M1/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL23/MPIF-1

CCL23, also known as MPIF-1, Ck beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the CCL23 gene results in two mRNAs that encode a short (Ck beta 8) and a long (Ck beta 8-1) isoform of the chemokine. Ck beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature Ck beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue Ck beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, Ck beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, CCL23 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). CCL23 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. CCL23 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. CCL23 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, osteoclast precursors.

References
  1. Patel, V. et al. (1997) J. Exp. Med. 185:1163.
  2. Youn, B-S. et al. (1998) Blood 91:3118.
  3. Nardelli, B. et al. (1999) J. Leukoc. Biol. 61:822.
  4. Berkhout, T.A. et al. (2000) Biochem. Pharmacol. 59:591.
Entrez Gene IDs
6368 (Human)
Alternate Names
CCL23; CCL23/MPIF-1; MPIF1; MPIF-1

Citation for Recombinant Human CCL23/MPIF-1 (aa 46-120) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Proinflammatory proteases liberate a discrete high-affinity functional FPRL1 (CCR12) ligand from CCL23.
    Authors: Miao Z, Premack BA, Wei Z, Wang Y, Gerard C, Showell H, Howard M, Schall TJ, Berahovich R
    J. Immunol., 2007;178(11):7395-404.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay

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