Recombinant Human CD109 Protein, CF Summary
Val22-Ser1268 (Tyr703Ser & Thr1241Met), with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares71% - 73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27% - 33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5 - 9). It is produced as a 170 kDa - 180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).
- Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
- Lin, M. et al. (2002) Blood 99:1683.
- Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
- Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
- Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
- Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
- Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
- Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
- Hashimoto, M. et al. (2004) Oncogene 23:3716.
- Solomon, K.R. et al. (2004) Gene 327:171.
- Finnson, K.W. et al. (2006) FASEB J. 20:1525.
- Schuh, A.C. et al. (2002) Blood 99:1692.
- Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
- Rozman, P. (2002) Transpl. Immunol. 10:165.
Citation for Recombinant Human CD109 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity
Authors: P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen
JCI Insight, 2021;6(9):.
Sample Types: Whole Cells
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