Recombinant Human CD160 Protein, CF

Catalog # Availability Size / Price Qty
6177-CD-050

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Recombinant Human CD160 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When recombinant mouse HVEM Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of recombinant human CD160 that produces 50% of the optimal binding response is found to be approximately 0.1 ‑ 0.6 μg/mL.

Source
Chinese Hamster Ovary cell line, CHO-derived human CD160 protein
Ile27-Ser159, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ile27
Predicted Molecular Mass
15.6 kDa
SDS-PAGE
22-30 kDa, reducing conditions

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6177-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6177-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD160

CD160 (also Natural killer cell receptor BY55) is a 27 ‑ 30 kDa member of the Ig superfamily (1 ‑ 4). In human, it is expressed principally on nonmyeloid hematopoietic cells. These include CD56DIMCD16+ cytolytic NK cells, CD8+CD28- T cells, CD8+CD101+ IELs, NKT cells, gamma δ TCR T cells, activated CD4+ T cells, and vascular endothelial cells (1, 5 ‑ 7). CD160 was initially identified as a GPI‑linked glycoprotein (3). It is synthesized as a preproprotein that is 181 amino acids (aa) in length. The precursor contains a 26 aa signal sequence, a 133 aa mature molecule that shows one 96 aa V‑type Ig‑like domain (aa 27 ‑ 122), and a 22 aa prosegment that is cleaved to generate a GPI‑linkage at Ser159. GPI‑linked CD160 is known to be cleaved by phospholipases and these generate an 80 kDa (presumably trimeric) band in SDS‑PAGE (1, 8). Alternative splice forms for CD160 are reported to exist on activated NK cells. The principal variant is an extended type I transmembrane (TM) protein that shows a 55 aa substitution for the C‑terminal two amino acids. It contains a 23 aa TM segment (aa 160 ‑ 182) and a 52 aa cytoplasmic region. Two other variants show deletions of the Ig‑like domain in both the GPI‑linked and TM form (9). Mature human CD160 shares 62% aa identity with mouse CD160.

CD160 is known to bind to HLA-G1, HLA-C, and HVEM (6, 9, 10). And upon engagement, it is reported to associate with CD2 in cis under certain conditions (11, 12). The effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on CD56DIM NK cells, CD160 signaling in response to HLA-C binding promotes IFN-gamma, TNF-alpha, and IL-6 secretion (10). And when up‑regulated on CD4+ T cells following activation, CD160 engagement by HVEM (expressed by APC) serves to block a simultaneous LIGHT stimulation of HVEM that promotes receptor expression and cytokine release (1, 2, 7, 13).

References
  1. Cai, G. & G.J. Freeman (2009) Immunol. Rev. 229:244.
  2. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
  3. Maiza, H. et al. (1993) J. Exp. Med. 178:1121.
  4. Anumanthan, A. et al. (1998) J. Immunol. 161:2780.
  5. Abecassis, S. et al. (2007) J. Invest. Dermatol. 127:1161.
  6. Fons, P. et al. (2006) Blood 108:2608.
  7. Kaye, J. et al. (2008) Nat. Immunol. 9:122.
  8. Giustiniani, J. et al. (2007) J. Immunol. 178:1293.
  9. Giustinani, J. et al. (2009) J. Immunol. 182:63.
  10. Barakonyi, A. et al. (2004) J. Immunol. 173:5349.
  11. Nikolova, M. et al. (2002) Int. Immunol. 14:445.
  12. Rabot, M. et al. (2006) Transpl. Immunol. 17:36.
  13. Cai, G. et al. (2008) Nat. Immunol. 9:176.
Entrez Gene IDs
11126 (Human); 54215 (Mouse); 502585 (Rat); 102117412 (Cynomolgus Monkey)
Alternate Names
BY55; BY55FLJ46513; CD160 antigenimmunoglobulin superfamily member; CD160 molecule; CD160; Natural killer cell receptor BY55; NK1; NK28

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