Recombinant Human CD200R1 Fc Chimera Protein, CF

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3414-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human CD200R1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD200 R1 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) that produces 50% of the optimal binding response is appoximately 2.5-15 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human CD200 R1 protein
Human CD200 R1
(Ala27-Leu266)
Accession # AAQ19772
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
53.4 kDa (monomer)
SDS-PAGE
85-95 kDa, reducing conditions

Product Datasheets

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3414-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3414-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily (1-3). The standard human CD200 R1 cDNA encodes a 325 amino acid (aa) precursor that includes a 28 aa signal sequence, a 215 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, human CD200 R1 shares 56% aa sequence identity with mouse and rat CD200 R1. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted. The protein expressed here contains a mature region that is identical to that of the standard form. There is an N-terminal extension of 25 aa that, in the standard form, is part of the signal sequence. In human, a separate CD200 R12 gene encodes a protein that shares 81% ECD aa identity with CD200 R11. In mouse, at least four genes for CD200 R1-like molecules have been described (4‑6). CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (7 - 9), while its ligand, CD200, is widely distributed (10). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (11). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (12). The capacity of CD200 R1-like molecules to interact with CD200 is controversial (5, 13). CD200 R1 propagates inhibitory signals despite its lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (8, 9, 14, 15) CD200 R1-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 6).

References
  1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
  2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  4. Wright, G.J. et al. (2003) J. Immunol. 171:3034.
  5. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  6. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
  7. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  8. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  9. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  10. Wright, G.J. et al. (2001) Immunology 102:173.
  11. Hoek, R.M. et al. (2000) Science 290:1768.
  12. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  13. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  14. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  15. Zhang, S. et al. (2004) J. Immunol. 173:6786.
Long Name
CD200 Receptor 1
Entrez Gene IDs
131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)
Alternate Names
CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor

Citation for Recombinant Human CD200R1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mechanistic dissection of the PD-L1:B7-1 co-inhibitory immune complex
    Authors: SC Garrett-Th, A Massimi, EV Fedorov, JB Bonanno, L Scandiuzzi, B Hillerich, RD Seidel, JD Love, SJ Garforth, C Guha, SC Almo
    PLoS ONE, 2020-06-04;15(6):e0233578.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human CD200 R1 Fc Chimera Protein, CF
By Anonymous on 02/07/2018
Application: Binding assay/Protein-protein interaction
Reason for Rating: Binding to CD200R observed by SPR.