Recombinant Human CD23/Fc epsilon RII Protein, CF Summary
Asp48-Ser321, with an N-terminal 9-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: CD23/Fc epsilon RII
CD23 (also named B cell differentiation antigen) is a member of subgroup II of the C-type (Ca++-dependent) lectin superfamily (1 - 5). Human CD23 is a 47 kDa, type II transmembrane glycoprotein that is expressed by a wide variety of cell types (6 - 10). The full-length receptor is 321 amino acids (aa) in length and contains a 274 aa extracellular region, a 26 aa transmembrane segment, and a 21 aa cytoplasmic domain. The extracellular region contains a C-type lectin domain and a connecting stalk with coiled-coil topography (3, 11). The lectin domain binds both protein and carbohydrate in an apparently Ca++ independent manner (11). The coiled-coil region contributes to oligomerization (11, 12). The lectin domain in human CD23 (aa 162 - 284) is 64%, 62% and 68% aa identical to the lectin domains in mouse, rat and bovine CD23, respectively. In the cytoplasmic region, two FC isoforms exist which arise from alternate start sites (6, 12). The “a” (or long) isoform begins with the sequence MEEGQYS and is constitutively expressed by B cells. It is believed to participate in IgE-mediated endocytosis (13). The “b” (or short) isoform begins with MNPPSQ and is induced on a wide variety of cell types by IL-4 (6). Fcb reportedly contributes to IgE-mediated phagocytosis (13). Fcb expressing cells include eosinophils, monocytes, visceral smooth muscle and intestinal epithelium (6, 14, 15). At least four soluble forms of CD23 are known to exist. They range in molecular weight from 25 kDa to 37 kDa, with the 25 kDa form predominating in sera (16). Soluble CD23 (sFc) is generated by metalloprotease (ADAM8; ADAM15; ADAM28) and cysteine-protease activity (16 - 18). Cleavage usually occurs between aa 150 - 160 (7, 8). It is unclear if sequential metalloprotease-cysteine protease activity is necessary for the generation of all soluble forms. Both soluble and membrane-bound CD23 show bioactivity. Ligands for CD23 include CD21, IgE, CD11b, and CD11c (19 - 21). CD23 binding to CD11b and Cd11c on monocytes results in oxidative product generation and proinflammatory cytokine release (21). On B cells, sCD23 induces IgE secretion by binding CD21. Conversely, secreted IgE will, in turn, bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (11).
- Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648.
- Heyman, B. (2000) Annu. Rev. Immunol. 18:709.
- Bajorath, J. and A. Aruffo (1996) Protein Sci. 5:240.
- Drickamer, K. (1993) Curr. Opin. Struct. Biol. 3:393.
- Drickamer, K. (1999) Curr. Opin. Struct. Biol. 9:585.
- Yokota, A. et al. (1988) Cell 55:611.
- Ludin, C. et al. (1987) EMBO J. 6:109.
- Ikuta, K. et al. (1987) Proc. Natl. Acad. Sci. USA 84:819.
- Kikutani, H. et al. (1986) Cell 47:657.
- Letellier, M. et al. (1988) J. Immunol. 141:2374.
- Hibbert, R.G. et al. (2005) J. Exp. Med. 202:751.
- Beavuil, A.J. et al. (1992) Proc. Natl. Acad. Sci. USA 89:753.
- Yokota, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:5030.
- Belleau, J.T. et al. (2005) Clin. Mol. Allergy 3:6.
- Tu, Y. et al. (2005) Gastroenterology 129:928.
- Marolewski, A.E. et al. (1998) Biochem. J. 333:573.
- Fourie, A.M. et al. (2003) J. Biol. Chem. 278:30469.
- Karagiannis, S.N. et al. (2001) Immunology 103:319.
- Aubry, J-P. et al. (1992) Nature 358:505.
- Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195.
- Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.
Citations for Recombinant Human CD23/Fc epsilon RII Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Relationship between serum inhibitory activity for IgE and efficacy of Artemisia pollen subcutaneous immunotherapy for allergic rhinitis: a preliminary self-controlled study
Authors: W Wang, J Yin, X Wang, T Ma, T Lan, Q Song, Y Guo
Allergy Asthma Clin Immunol, 2020;16(0):18.
Sample Types: Cell Culture Lysates
Applications: ELISA Capture
The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab
Authors: P Gasser, SS Tarchevska, P Guntern, D Brigger, R Ruppli, N Zbären, S Kleinboelt, C Heusser, TS Jardetzky, A Eggel
Nat Commun, 2020;11(1):165.
Sample Types: Complex Sample Type
Applications: ELISA Capture
Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy
Authors: C Fukano, K Ohashi-Doi, K Lund, A Nakao, K Masuyama, T Matsuoka
J. Pharmacol. Sci., 2019;0(0):.
Sample Types: Serum
Applications: Bioassay, ELISA Capture
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